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Strands

strand

(Science: geography) The shore, especially the beach of a sea, ocean, or large lake; rarely, the margin of a navigable river. Strand birds.

(Science: zoology) The brown hyena.

Origin: AS. Strand; akin to D, G, Sw, & Dan. Strand, Icel. Strond.


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Help: Confused with primers in a PCR of a vector?

... to just determine the directionality of our insert. Because later in the lab, we did a whole bunch of sequence alignments with our gene (of both strands) and BLASTed it. But...I am just not really sure if I've done the PCR reactions correctly. Any help would be greatly appreciated. Thanks!

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

Re: mRNA Search for RT-PCR (U to T)

If we design primers to one strand out of two strands of cDNA then there is a chance that one of the primer will not be complementary to mRNA and it will not bind to mRNA.How can we synthesize cDNA from that mRNA?

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by microniranjan
Sat Oct 05, 2013 9:58 am
 
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 4243

DNA helix and gene

... other, so one gene sequence can be found on one strand and the second gene sequence on another (read in the opposite direction). However, both strands are required for transcription to mRNA since it's the complement strand that is used as template for it...

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by Cat
Sun Aug 18, 2013 4:36 pm
 
Forum: Molecular Biology
Topic: DNA helix and gene
Replies: 2
Views: 1987

DNA helix and gene

Do the two strands of DNA helix carry the same genetic information?

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by Fearnot
Sat Aug 17, 2013 10:30 am
 
Forum: Molecular Biology
Topic: DNA helix and gene
Replies: 2
Views: 1987

Please comment this theory, is this theory correct?

... same time. 2) The water is everywhere, but the idea is that DNA is hydrophobic so it pushes the water away. However, when it divides, the 2 DNA strands seperate and then it loses it's hydrophobic properties. My theory is put online on my website yes and I put it here too. If it may help cancer ...

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by schang1984
Thu Aug 15, 2013 4:12 pm
 
Forum: Cell Biology
Topic: Please comment this theory, is this theory correct?
Replies: 5
Views: 2651
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