Stock

... -70°C.) Solutions: RF1 Compound (end concentration) Amount for 100ml RbCl (100mM) 1.2g MnCl2•4H2O (50mM) 0.998g Potassium acetate (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with dilute acetic acid. Don’t ...

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Does anyone have areliable protocol for thawing cells from frozen stock? thanks

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... incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately. Since I have kept my original stock of cells before making them competent, I did the same test on the original stack and i saw colonies only on the tetracyclin plates (XL1 Blue is ...

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... in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume to work with? lets say I need 10 ml of this ...

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... my reading, it says that i can use 6.7% DMSO or 1.25% Formamide. but i'm confuse the % is from the total PCR reaction or from the concentration of stock. Do you guys have any idea? Thank you.

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