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Stock

stock

group of organisms (all of the same species) that is genetically self-sustaining and isolated geographically or temporally during reproduction. A special variety of domesticated animals within a species; he experimented on a particular breed of white rats; he created a new strain of sheep.


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Problems making chemically competent cells

... -70°C.) Solutions: RF1 Compound (end concentration) Amount for 100ml RbCl (100mM) 1.2g MnCl2•4H2O (50mM) 0.998g Potassium acetate (30mM) 3ml (1M stock pH 7.5, 9.814g in 100ml solution, pH with acetic acid) CaCl2•2H2O (10mM) 0.15g Glycerol (15% w/v) 15g Final pH: 5.8 with dilute acetic acid. Don’t ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 354

thaw cells

Does anyone have areliable protocol for thawing cells from frozen stock? thanks

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by Bio2013
Thu Jan 31, 2013 7:48 pm
 
Forum: Molecular Biology
Topic: thaw cells
Replies: 1
Views: 189

Competent cell growth on all my plates (multi resistant)

... incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately. Since I have kept my original stock of cells before making them competent, I did the same test on the original stack and i saw colonies only on the tetracyclin plates (XL1 Blue is ...

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by emadsadeghian
Fri Jan 04, 2013 12:27 pm
 
Forum: Molecular Biology
Topic: Competent cell growth on all my plates (multi resistant)
Replies: 0
Views: 377

The same old silly question about molarities

... in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume to work with? lets say I need 10 ml of this ...

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by bravebeaker
Wed Dec 26, 2012 3:35 am
 
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 502

How to use PCR enhancer

... my reading, it says that i can use 6.7% DMSO or 1.25% Formamide. but i'm confuse the % is from the total PCR reaction or from the concentration of stock. Do you guys have any idea? Thank you.

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by elynn
Thu Dec 20, 2012 2:48 am
 
Forum: Molecular Biology
Topic: How to use PCR enhancer
Replies: 1
Views: 411
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