Sticky ends

... already are. How long it would take an isolated genome to speciate depends on its genetic learning rate (how fast it gains or changes new information/genes). ... at each end of the 2 supercoiled chromosomes that fused became sticky by removal of the repeating code that forms a protective layer ...

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... good efficiency (the reason is mainly that bases can be lost at the ends, or that it can ligate and produce a molecule that is missing a few ... then I go back and CIP the vector and it usually helps. Unfortunately sticky ends are not as foolproof as textbooks make you think they are. 3) ...

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It's not to prevent re-ligation -- the sticky ends are compatible and would potentially re-ligate anyway. Rather, there might not be one restriction ...

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... might need two restrictino enzymes to cut the plasmid to present sticky ends suitable for the dna fragment to be inserted in..is my understanding correct?i'm an undergrad ...

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... a different restriction enzyme to cut the plasmid, one the presents sticky ends that are compatible with those on the fragment, at least presents a possible strategy ...

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