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Sticky ends

(noun) Plural form of sticky end.

See sticky end for definition and additional information.


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Telomere and DNA replication

... that telomere binding proteins prevent exonucleases degrading the sticky ends of the parent strand. As such when the second generation of cells divides the third generation ...

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by jsmith613
Sun Oct 06, 2013 11:10 pm
 
Forum: Genetics
Topic: Telomere and DNA replication
Replies: 0
Views: 1241

Campylobacter RFLP troubleshooting

... amplify your GOI and then cut it with RE for what exactly? Just at the ends to make sticky ends for consequent cloning? And I guess you use your PCR reaction without any purification?

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by JackBean
Sun May 05, 2013 3:54 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1973

ligation problem although try everything,suggestions?

... my insert after PCR with the enzymes which my insert have at it's ends (verify again with agarose gel) . Alternatively I cloned my insert to ... there. I digested my vector with the insert's same enzymes and make sticky(cohesive) end ligation. I used Quick ligase at room temperature(25 ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4163

dna ligation protocol for cohesive end

... use 16-17°C for O/N. The point of such low temperature is that the sticky ends are short thus the pairing is not much efficient.

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by JackBean
Mon Jan 30, 2012 11:09 am
 
Forum: Molecular Biology
Topic: dna ligation protocol for cohesive end
Replies: 3
Views: 1743

genetic engineering- not understanding some concepts

... solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you know any sites that have answers as well as questions ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1777
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