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Sticky end

Definition

noun, plural: sticky ends

(molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the terminal portion has a stretch of unpaired nucleotides, and the strands are not of the same length.


Supplement

The ends of the DNA are sticky because it can easily match up with a complementary overhang.

For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region:

5'-ATCTGACT ______+ GATGCGTATGCT-3'
3'-TAGACTGACTACG________CATACGA-5'


Synonym: DNA end, cohesive end.
Compare: blunt end.
See also: restriction enzyme, overhang (molecular biology).


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... let it run its course. I've had it twice, now. The first time was a couple weeks ago. My vomit and diarrhea were very dark brown with a seemingly sticky consistency. I only vomitted twice (in one sitting) with the first time. This second time from which I am currently recovering I was dry heaving ...

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Wait a sec. So you amplify your GOI and then cut it with RE for what exactly? Just at the ends to make sticky ends for consequent cloning? And I guess you use your PCR reaction without any purification?

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by JackBean
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... ratio between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation ...

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