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Sticky end

Definition

noun, plural: sticky ends

(molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the terminal portion has a stretch of unpaired nucleotides, and the strands are not of the same length.


Supplement

The ends of the DNA are sticky because it can easily match up with a complementary overhang.

For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region:

5'-ATCTGACT ______+ GATGCGTATGCT-3'
3'-TAGACTGACTACG________CATACGA-5'


Synonym: DNA end, cohesive end.
Compare: blunt end.
See also: restriction enzyme, overhang (molecular biology).


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Re: MOLECULAR CLONING

... ratio between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 4103

pine resin problem

... studio, and last Christmas I somehow brilliantly managed to get it all sticky with pine resin. I was driving the tree to my house, so my car got ... either clean everything (impossible), or wait for resin to come to the end of it's life cycle and completely dry; then it won't be a problem. I ...

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by jan31
Sat Jun 09, 2012 12:50 pm
 
Forum: Botany Discussion
Topic: pine resin problem
Replies: 3
Views: 1911

ligation problem although try everything,suggestions?

... my insert after PCR with the enzymes which my insert have at it's ends (verify again with agarose gel) . Alternatively I cloned my insert to ... there. I digested my vector with the insert's same enzymes and make sticky(cohesive) end ligation. I used Quick ligase at room temperature(25 ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 3271

Designing primers to clone into expression vector

... primers, which cover a restriction site which can be cut to create a sticky end which I can use to insert my protein into a pet vector? Thanks!

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by Jemi
Wed Feb 15, 2012 8:11 pm
 
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 1464

dna ligation protocol for cohesive end

yes,sticky end ligation. In some protocol it says keep in 16 °C overnight,some says 20°C 4 hours and some says 4°C .I am mixed ...

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by genetherapy
Fri Jan 27, 2012 3:54 pm
 
Forum: Molecular Biology
Topic: dna ligation protocol for cohesive end
Replies: 3
Views: 1525
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