Steric

Lay a steric-blocking oligo across the SNP and see if splicing changes.

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... ). This isn't a change in DNA, but an alteration of the splicing of pre-mRNA caused by dosing with a steric-blocking antisense oligo. It is a nucleic acid "operation", but perhaps not a "genetic operation" as it changes a gene transcript ...

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... of print] Abes R, Moulton HM, Clair P, Yang ST, Abes S, Melikov K, Prevot P, Youngblood DS, Iversen PL, Chernomordik LV, Lebleu B. Delivery of steric block morpholino oligomers by (R-X-R)4 peptides: structure-activity studies. Nucleic Acids Res. 2008 Sep 16. [Epub ahead of print] Bok K, Cavanaugh ...

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... attraction and hydrogen-bonding of the guanidiniums to the phosphate groups of phospholipids. Moulton HM, Moulton JD. Peptide-assisted delivery of steric-blocking antisense oligomers. Curr Opin Mol Ther. 2003 Apr;5(2):123-32. We think that a similar mechanism assists delivery of the Vivo-Morpholinos ...

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... rate of reaction depends on (using collision theory): Rate = kl[A][C] = pfZ[A][C] (I had to use C instead of because of BOLD code) where, p = the steric factor (or the fraction of collisions properly oriented for reaction) f = the fraction of collisions that exceed Arrhenius activation energy ...

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