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Dictionary » S » Step Stepstep 1. In dentistry, a dove-tailed or similarly shaped projection of a cavity prepared in a tooth into a surface perpendicular to the main part of the cavity for the purpose of preventing displacement of the restoration (filling) by the force of mastication. 2. A change in direction resembling a stair-step in a line, a surface, or the construction of a solid body. 3. To move the foot in walking; to advance or recede by raising and moving one of the feet to another resting place, or by moving both feet in succession. 4. A prefix used before father, mother, brother, sister, son, daughter, child, etc, to indicate that the person thus spoken of is not a blood relative, but is a relative by the marriage of a parent; as, a stepmother to X is the wife of the father of X, married by him after the death of the mother of X. See Stepchild, Stepdaughter, Stepson, etc. ![]()
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Results from our forumTwo-color in situ helpProblem seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).
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TCA preipitation... at -20C So the supernatant will contain cell wall and the insoluble protoplast component will be left right? So is it the supernatant at the last step i TCA precipitate? if it is, i've looked at a protocol for TCA precipitation and if i followed that, i'd only be left with 2 components. One more ...
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Co2 from respiration-Sugars vs Fatslol idk, is the extra co2 just from pyruvate dehydrogenase step? so C18H36O18 vs stearic acid would only give 6 extra co2 since that would be (6) C3H6O3
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Problems making chemically competent cells... everything is cold. Any help here would be appreciated. I've pasted the exact method I use below this: • Pre-chill a flask on ice for the chilling step • Pre-chill the centrifuge and its rotor • Work in the cold room! • Pre-chill eppendorfs in the -70 freezer, and tips in the cold room, (and maybe ...
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