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Step

step

1. In dentistry, a dove-tailed or similarly shaped projection of a cavity prepared in a tooth into a surface perpendicular to the main part of the cavity for the purpose of preventing displacement of the restoration (filling) by the force of mastication.

2. A change in direction resembling a stair-step in a line, a surface, or the construction of a solid body.

3. To move the foot in walking; to advance or recede by raising and moving one of the feet to another resting place, or by moving both feet in succession.

4. A prefix used before father, mother, brother, sister, son, daughter, child, etc, to indicate that the person thus spoken of is not a blood relative, but is a relative by the marriage of a parent; as, a stepmother to X is the wife of the father of X, married by him after the death of the mother of X. See Stepchild, Stepdaughter, Stepson, etc.


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Two-color in situ help

Problem seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

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by vastgenome
Sat Mar 09, 2013 4:21 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 257

TCA preipitation

OHHH, do i TCA the s/n from step 3???

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by biology_06er
Wed Feb 27, 2013 3:38 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 159

TCA preipitation

... at -20C So the supernatant will contain cell wall and the insoluble protoplast component will be left right? So is it the supernatant at the last step i TCA precipitate? if it is, i've looked at a protocol for TCA precipitation and if i followed that, i'd only be left with 2 components. One more ...

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 159

Co2 from respiration-Sugars vs Fats

lol idk, is the extra co2 just from pyruvate dehydrogenase step? so C18H36O18 vs stearic acid would only give 6 extra co2 since that would be (6) C3H6O3

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by key
Thu Feb 14, 2013 5:04 am
 
Forum: Human Biology
Topic: Co2 from respiration-Sugars vs Fats
Replies: 12
Views: 792

Problems making chemically competent cells

... everything is cold. Any help here would be appreciated. I've pasted the exact method I use below this: • Pre-chill a flask on ice for the chilling step • Pre-chill the centrifuge and its rotor • Work in the cold room! • Pre-chill eppendorfs in the -70 freezer, and tips in the cold room, (and maybe ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 352
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