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Std

std --> sexually transmitted disease

(Science: disease) A disease, such as genital herpes, gonorrhoea, hIV or chlamydia, whose usual means of transmission is by sexual contact.


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Real time PCR

make sure to vortex your DNA samples before you mix them for the qPCR in order to generate the std curve. also make sure you measured the DNA accurately to make the std curve calculation, for example I found out that checking by nanodrop doesnt give me good results as ...

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by bravebeaker
Fri Oct 19, 2012 6:26 am
 
Forum: Molecular Biology
Topic: Real time PCR
Replies: 4
Views: 2255

Re: hydroxyproline assay to determine collagen content

thanks Jack, here is my protocol: 50ul of test samples containing Hyp (10ug/ml) and aliquots of std hyp (2-20ug) prepared from 10mg/ml stock, this is then hydrolysed with 50ul of 2N NaOH (2N final conc) at 120 degree C for 20 min. To this add 450ul of Chloramine T and oxidise ...

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by Stip
Wed Aug 03, 2011 6:15 am
 
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 8042

1 day period

... that i should wait to see if the rest of my period comes though out the month & it did last month and this month. i've already been tested for STD/STI's & im clear, i have never had anything :) Nipples are fair sore today- Boyfriend keeps joking that im pregnant. Its not funny :')

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by Blackberry12
Wed Feb 23, 2011 4:57 pm
 
Forum: Human Biology
Topic: 1 day period
Replies: 18
Views: 10249

Re: Stomach bloating and weight gain

... as when I was a size 2. I have had a ct scan of my abdomen and brain, extensive bloodwork including thyroid tests, internal/external ultrasound, std tests, spinal x-ray. They now have me on miralax to check if it is possibly IBS, if not they are going to do laproscopic surgery to check for endometriosis. ...

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by amylv23
Wed Jun 09, 2010 6:25 am
 
Forum: Physiology
Topic: Stomach bloating and weight gain
Replies: 453
Views: 2703288

Re: Stomach bloating and weight gain

... again request that I have a xray and/or ultrasound b/c this is the first time I have ever experienced something like this. I am straight on being STD free so that's not my problem. But thanks to alot of you on this website with the knowlegable advice. I will be asking questions about IBS, gallstones, ...

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by Shaunypooh2008
Wed Oct 15, 2008 9:22 pm
 
Forum: Physiology
Topic: Stomach bloating and weight gain
Replies: 453
Views: 2703288
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