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Start codon

start codon --> initiation codon

The codon 5' AUG in mRNA, at which polypeptide synthesis is started. It is recognised by formylmethionyl tRNA in bacteria and by methionyl tRNA in eukaryotes.


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Gene cloning question

Thank you. Interesting argument on the extra-Kozak and start codon... So is it even better to leave a few extra amino acids as for a linker in a fusion protein? ...

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by kk
Mon Aug 03, 2009 9:44 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Gene cloning question

you wouldn't want to leave the Kozac sequence and start codon in between the two coding sequences just incase the genes should spit, which there is a chance ...

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by Eous
Mon Aug 03, 2009 2:57 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Two questions about operon (transcription/translation)

... for each open reading frame. However, others do not: sometimes the last codon of one open reading frame coincides with the first codon of another ... finished translating one reading frame it is in an ideal position to start translating the next reading frame, making it unnecessary to have another ...

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by MrMistery
Sun Aug 02, 2009 11:27 pm
 
Forum: General Discussion
Topic: Two questions about operon (transcription/translation)
Replies: 2
Views: 106

Gene cloning question

... reporter gene), does it matter if you leave the Kozak sequence and a start codon in between the two coding regions? If it's OK how come that there is no reporter protein ...

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by kk
Wed Jul 29, 2009 8:07 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Re: Tricky translation question, can someone help please?

... proteins bind the DNA and help a polymerase to hook onto the DNA and start transcription. You need to start with a methionine codon (AUG) and then count triplets (including the methionine) until you reach the stop (which ...

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by jonmoulton
Mon Jun 15, 2009 11:29 pm
 
Forum: Molecular Biology
Topic: Tricky translation question, can someone help please?
Replies: 1
Views: 131
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