Start codon

Thank you. Interesting argument on the extra-Kozak and start codon... So is it even better to leave a few extra amino acids as for a linker in a fusion protein? ...

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you wouldn't want to leave the Kozac sequence and start codon in between the two coding sequences just incase the genes should spit, which there is a chance ...

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... for each open reading frame. However, others do not: sometimes the last codon of one open reading frame coincides with the first codon of another ... finished translating one reading frame it is in an ideal position to start translating the next reading frame, making it unnecessary to have another ...

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... reporter gene), does it matter if you leave the Kozak sequence and a start codon in between the two coding regions? If it's OK how come that there is no reporter protein ...

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... proteins bind the DNA and help a polymerase to hook onto the DNA and start transcription. You need to start with a methionine codon (AUG) and then count triplets (including the methionine) until you reach the stop (which ...

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