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Start codon

start codon --> initiation codon

The codon 5' AUG in mRNA, at which polypeptide synthesis is started. It is recognised by formylmethionyl tRNA in bacteria and by methionyl tRNA in eukaryotes.


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Re: Any ideas for vaccine development or anti-viral drugs???

... infection. A 22-mer P-PMO designed to base pair with the translation start site region of EBOV VP35 positive-sense RNA generated sequence-specific ... mice treated with a PMO complementary to a region spanning the start codon of VP24 mRNA were protected against lethal Ebolavirus challenge. In ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 478

Gene cloning question

... don't you? However if I were you, I'd be rather concerned about the start codon of the N-terminal fusion.

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by JackBean
Fri Feb 07, 2014 9:38 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 17918

Re: Gene cloning question

... that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 17918

How can I add a kozak sequence in plasmid pMT/bip/V5-His A

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life ...

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by JackBean
Wed Mar 13, 2013 4:59 pm
 
Forum: Molecular Biology
Topic: How can I add a kozak sequence in plasmid pMT/bip/V5-His A
Replies: 1
Views: 2096

Re:

... (in reality it's much longer). In such case, you want your primer to start at GTC and go on further. By lipoprotein motif he probably meant whether ... me was: ATG NNN NNN...1300 NT long. There is nothing before the start codon. So, I assume that implies there is no signal peptide? Also, my forward ...

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by coppcw08
Mon Nov 05, 2012 12:27 am
 
Forum: Molecular Biology
Topic: Sequencing question/primer design
Replies: 4
Views: 3270
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