Start codon

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life ...

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... (in reality it's much longer). In such case, you want your primer to start at GTC and go on further. By lipoprotein motif he probably meant whether ... me was: ATG NNN NNN...1300 NT long. There is nothing before the start codon. So, I assume that implies there is no signal peptide? Also, my forward ...

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... You end up with a few extra amino acids added in just after the start of translation. When you clone into the polylinker, you may or may ... many more amino acids (assuming you don't clone in an in-frame stop codon).

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Ane I was trying to point out also, that the START codon AUG is part of the gene ;)

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... transcription initiation of gene 2? Or can i go straight from the stop codon of gene 1 to the start codon of gene 2? Thanks for your help! Labfairy

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