Standardize

... because I was not able to find resources that would help me make the decision and back it up. We lyse the cells and run WB and would like to standardize to one control, either 293 or 293TN. Any information is greatly appreciated. Thank you.

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The methods are already standardize. However, it seems that every time we carry out an experiment, the fluorescence readings that we obtain seems to deviate around a few hundreds to a thousand. Although this difference is unanimous throughout ...

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... differences -> the batch is not the same and your experimental conditions vary because of that. Chemical reactions are usually much easier to standardize, but even these can give a bit different outocomes if you add a couple of milligrams more of Reagent X to the batch 2 than you did with ...

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... use net amount created i have an idea. do you know if water content somehow affect the amounts of the substance created. would rather suggest you standardize the weights and amounts of water. and carry out the experinebt again else do not use conc. and do as i told. hope that helps best of luck

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... the outcome. That being said, it does give a good rough estimation of cell numbers, which is often well enough. Also, if you do it a lot, you standardize your techniques, and you have the patience to count enough of cells, you can get quite accurate numbers as well. Just don't go and compare ...

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