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Standard volume

standard volume

The volume of an ideal gas at standard temperature and pressure, approximately 22.414 liters.


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Re: FLU/time to FLU/mg/min

... in mM is supposed to be obtained by comparing the enzyme data with the standard curve in such a way that I convert the RFU (for the enzyme data) ... it is the molecualr weight of the fluorescent is I am right? 2) what volume i will be using or referring to is it the total volume of the reaction ...

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by dalin
Mon Nov 05, 2012 8:10 am
 
Forum: General Discussion
Topic: FLU/time to FLU/mg/min
Replies: 6
Views: 2773

EC50 depending on cell number?

... it actually very much not only depends on the cell number but also the volume. I think this is wrong but I cannot really explain why it is wrong. ... a compound has an e.g. EC50 of 1 mM (so a quite inactive compound) in a standard assay with 10.000 cells per well with 100ul medium volume. If theoretically ...

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by qforthemass
Tue Aug 14, 2012 9:40 pm
 
Forum: Cell Biology
Topic: EC50 depending on cell number?
Replies: 1
Views: 2913

Re: hydroxyproline assay to determine collagen content

... 14% the amino acids in collagen. Materials: Hydroxyproline Standard - 1-5 µg/2 0.001N HCl ml. Buffer-0.5 g citric acid monohydrate, ... anhydrous, and 0.34 g sodium hydroxide are combined and the final volume is brought to 10 ml with distilled water. Make sure the pH is 6.0. ...

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by ucsfmed2012
Thu Oct 27, 2011 9:30 am
 
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 8048

equal amounts of total protein

... in taking equal amounts of protein. For this purpose I took relevant volume from each sample (volume that contains same protein amounts), dissolved ... the following distribution between the samples: Average = 265.9 ug/ml Standard Deviation (SD) = 25.0 Min = 213.2 Max = 332.6 My question is whether ...

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by fth1
Thu Apr 21, 2011 2:21 pm
 
Forum: Molecular Biology
Topic: equal amounts of total protein
Replies: 2
Views: 1389

Pipetting small volumes

For very small volume pipettes with disposable plastic tips are not reliable. The metal pipette like those from Hamilton: http://www.hamiltoncompany.com are the standard. They are not cheap, but well cared for they can last a very long time. There are probably ...

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by canalon
Tue Sep 14, 2010 7:07 pm
 
Forum: General Discussion
Topic: Pipetting small volumes
Replies: 3
Views: 2886
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