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Staining

staining

The use of a dye, reagent, or other material for producing colouration in tissues or microorganisms for microscopic examination.


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Will antibody staining work on mouse quad sections

I stained sections with hematoxylin first and now want to do antibody staining. Will it work?

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by 2444256703
Wed Mar 11, 2015 8:32 am
 
Forum: General Discussion
Topic: Will antibody staining work on mouse quad sections
Replies: 0
Views: 124

Methylene Blue Solution use in staining variety of bacteria?

... Blue Solution (Loeffler's) is used in various procedures such as the Ziehl-Neelsen, Fite's, and Gram stain. The stain is also suggested for use in staining a variety of bacteria (Corynebacterium diphtheria, Haemophilus influenzae, Neisseria, etc.) and leukocytes. This reagent produces dark blue ...

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by scytek
Sun Feb 22, 2015 9:22 am
 
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 268

separation problem with GelRed

... with a lower percentage gel. 4. Change the running buffer. TBE buffer has a higher buffering capacity than TAE buffer. 5. Try using the post-staining protocol if possible, especially if more than the recommended amount of DNA is required. For other questions regarding GelRed, check out Biotium’s ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19558

mycoplasma testing

... been testing for mycoplasma till now, but would like to start. We were thinking of using PCR based method as aprimary method and vero cells staining as a second method. not sure how often and what lines I need to test (we have fibroblasts and neuronal cultures). does anyone have a example ...

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by revi
Tue Feb 25, 2014 11:03 am
 
Forum: Molecular Biology
Topic: mycoplasma testing
Replies: 0
Views: 1093

Re: Re:

... might be in order. So in a nutshell: I cannot rule out the presence of bacteria for certain, but the findings in your blood film can also be staining artifacts or (bacterial) contamination. This is important to note, since especially some species of Bartonella can indeed cause persistent ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 15876
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