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Staining

staining

The use of a dye, reagent, or other material for producing colouration in tissues or microorganisms for microscopic examination.


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mycoplasma testing

... been testing for mycoplasma till now, but would like to start. We were thinking of using PCR based method as aprimary method and vero cells staining as a second method. not sure how often and what lines I need to test (we have fibroblasts and neuronal cultures). does anyone have a example ...

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by revi
Tue Feb 25, 2014 11:03 am
 
Forum: Molecular Biology
Topic: mycoplasma testing
Replies: 0
Views: 107

Re: Re:

... might be in order. So in a nutshell: I cannot rule out the presence of bacteria for certain, but the findings in your blood film can also be staining artifacts or (bacterial) contamination. This is important to note, since especially some species of Bartonella can indeed cause persistent ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3425

Re: Re:

... might be in order. So in a nutshell: I cannot rule out the presence of bacteria for certain, but the findings in your blood film can also be staining artifacts or (bacterial) contamination. This is important to note, since especially some species of Bartonella can indeed cause persistent ...

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by biohazard
Fri Dec 13, 2013 8:13 am
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3425

What do you think these are, in my blood?

... I value my vet friend's opinion. He thinks these are a micrococci - perhaps Staphylococcus. What do you think of that? EDIT: Oh, btw, he said the staining is with the Wright modified stain.

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by snowathlete
Thu Dec 12, 2013 1:14 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3425

Re: Re:

... but I cannot say for certain from the images you posted. After all, a common Staphylococcus looks pretty much like a dark violet dot under most staining conditions. Though now as you said, there is an erythrocyte in the last panel (upper right-hand corner) that seems to have some staining that ...

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by biohazard
Tue Dec 10, 2013 2:02 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3425
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