SsRNA-RT

... forum but did not get a response, so I am re-posting this here ) For the viruses that must carry a specific enzyme (eg. viruses with ssDNA, -ssRNA genomes): 1. It is carried in the capsid AND coded for in their genomes, right? 2. since they need this enzyme before they can use their genome, ...

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For the viruses that must carry a specific enzyme (eg. viruses with ssDNA, -ssRNA genomes): 1. It is carried in the capsid AND coded for in their genomes, right? 2. since they need this enzyme before they can use their genome, how do they ensure that a lot is ...

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... how the protein are produced. You will see that the answers are slightly different for the different classes of RNA viruses, but that for dsRNA, +ssRNA and -ssRNA are a bit off the archetypal model, but still following the basic rule of the (now outdated) central dogma, that is the unique direction ...

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... method. This could be actually introducing an oligo into cells, such as one of the following: RNase-H competent antisense -- ssDNA oligos ssRNA oligos Phosphorothioate oligos Many sorts of chimeric oligos RNase-independent antisense -- Morpholino oligos 2'-O-methyl phosphorothioate oligos ...

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... the exception of small RNA which have self complimented and have been cleaved as we see with RNAi. When you perform an RT-PCR, you are using the ssRNA as a template and your resulting amplicon is a ssDNA known as cDNA. cDNA is always single stranded, with the exception of a DNA:RNA hybrid complex ...

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