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Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have a few questions. I guess after PCR amplification of the gene region of interest, I should use a sequencing primer that anneals to a region that has ...

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... i can do N-term sequencing. we don't have it here. i have abt 1.5mg of protein, ran on SDS-PAGE got a single band, but when i did 2DE i got 2 spots of the same MW but different pI. sent it for MALDI-ToF-ToF, this is the result i got. I was hoping that this protein would be a new protein. I ...

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"a virus was blown onto a gene" something is terribly odd with this statement same with "sun spots might have caused a lonely neutron to fly onto a gene" well this one is just wrong. Neutrons cant move so freely without some kind of catastrophic effect "chemicals ...

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... all those changes could/can be part of the random process that start mutations that generate new species ie a virus was blown onto a gene sun spots might have caused a lonely neutron to fly onto a gene chemicals in the atmosphere might have fallen onto a gene etc etc

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... all those changes could/can be part of the random process that start mutations that generate new species ie a virus was blown onto a gene sun spots might have caused a lonely neutron to fly onto a gene chemicals in the atmosphere might have fallen onto a gene etc etc that is a lot more than ...

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