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Sporulation

sporulation

(Science: biology) The act or process of forming spores; spore formation.


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How does pH change during sporulation? - Help

Hi folks, I carried out an experiment where I observed sporulation in a Bacillus species. The bacteria was innoculated in this medium that contained a bunch of salts, glucose, and ammonium acetate. Every few hours or so, we were required to take the ...

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by keetner
Mon Oct 22, 2012 10:22 pm
 
Forum: Microbiology
Topic: How does pH change during sporulation? - Help
Replies: 2
Views: 1582

Re: life cycle of buffing and fission yeast

... shortage triggers sexual reproduction. Haploid cells conjugate (diploid stage) and then perform meiosis giving four sexual haploid spores (sporulation). Traditional theory states that DNA recombination during meiosis helps withstand stress conditions by producing new gene combinations that ...

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by marquitosgm
Thu Mar 22, 2012 11:31 am
 
Forum: Cell Biology
Topic: life cycle of buffing and fission yeast
Replies: 8
Views: 3329

Re: sporulation in Bacillus

you might want to try to lower your temperature, and or time... or try to inactivate the vegetative cells with ethanol maybe these spores are not very heat resistant, or they germinate prior, or during your heating, wich would kill them. waht mutation is it 85┬░for 15 minutes incubated in wet heat (i...

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by cobaltbomb
Mon Nov 22, 2010 9:48 pm
 
Forum: Microbiology
Topic: sporulation in Bacillus
Replies: 1
Views: 1792

sporulation in Bacillus

Can anyone tell me about spores germination in B. pumilus? I am trying to count the sporulation efficiency in my B.pumilus mutants by phase contrast microscopy as well with counting on normal LB agar plate ( after heat shock at 80deg for 20min)..but the results in ...

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by praveen127
Thu Oct 07, 2010 3:08 pm
 
Forum: Microbiology
Topic: sporulation in Bacillus
Replies: 1
Views: 1792

cellophane

hi, I heard of a technique to improve sporulation in culture /germinate by using cellophane sheets on top of the agar plate. I am searching the web for such cellophane sheets that will fit a 90mm petri dish but can't find. anyone know where ...

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by rsg
Tue May 18, 2010 2:52 pm
 
Forum: Microbiology
Topic: cellophane
Replies: 3
Views: 2086
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