Sporulation

Hi folks, I carried out an experiment where I observed sporulation in a Bacillus species. The bacteria was innoculated in this medium that contained a bunch of salts, glucose, and ammonium acetate. Every few hours or so, we were required to take the ...

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... shortage triggers sexual reproduction. Haploid cells conjugate (diploid stage) and then perform meiosis giving four sexual haploid spores (sporulation). Traditional theory states that DNA recombination during meiosis helps withstand stress conditions by producing new gene combinations that ...

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you might want to try to lower your temperature, and or time... or try to inactivate the vegetative cells with ethanol maybe these spores are not very heat resistant, or they germinate prior, or during your heating, wich would kill them. waht mutation is it 85°for 15 minutes incubated in wet heat (i...

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Can anyone tell me about spores germination in B. pumilus? I am trying to count the sporulation efficiency in my B.pumilus mutants by phase contrast microscopy as well with counting on normal LB agar plate ( after heat shock at 80deg for 20min)..but the results in ...

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hi, I heard of a technique to improve sporulation in culture /germinate by using cellophane sheets on top of the agar plate. I am searching the web for such cellophane sheets that will fit a 90mm petri dish but can't find. anyone know where ...

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