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Split gene

split gene --> introns

Non-coding, intervening sequences of dNA that are transcribed, but are removed from within the primary gene transcript and rapidly degraded during maturation of messenger RNA. most genes in the nuclei of eukaryotes contain introns, as do mitochondrial and chloroplast genes.


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Genetics with Mitosis and Meiosis

... I got anything wrong. Anaphase (in any type of division) is after the genetic material has separated and is being pulled toward the ends of the ... II is basically like mitosis in that sister chromatids are being split apart, but there are half as many chromosomes (one from each homologous ...

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by wildfunguy
Sun Feb 03, 2013 11:45 pm
 
Forum: Genetics
Topic: Genetics with Mitosis and Meiosis
Replies: 2
Views: 3043

Re:

... there is some small alternative-intron in your exon, which can be thus split why are you using primers only for one exon at a time? Is your aim ... 4 sets of primers. I was going to amplify 4 overlapping regions of the gene, run them on a gel, cut out the bands of expected size, purify them, ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 1345

Real Time reverse transcription PCR Questions

... in mind is to transfect my cells with my desired proteins and when I split my cell lysate I will keep one for protein and one to isolate RNA. ... I know this is a dumb question but I’m having trouble locating my exact gene sequence! My project is on IL6. I used NCBI to search and found IL6 ...

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by wessH
Sun Jun 03, 2012 2:21 pm
 
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 1490

Molecular gene (genome) concept scientifically untenable

For nearly six decades now, biologists have been promoting molecular gene (DNA) as the blueprint of life. The world now believes that it is a chemical ... a single messenger RNA. Rather, most eukaryotic genes consist of split DNA sequences, often producing more than one mRNA by means of complex ...

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by PAWahid
Wed May 02, 2012 4:54 am
 
Forum: Molecular Biology
Topic: Molecular gene (genome) concept scientifically untenable
Replies: 12
Views: 14510

Re: Human evaluation

... (as with most of the human population), and are thus part of the same gene pool. I think this makes them unlikely to diverge/speciate unless the ... not speciate over the 50-60,000 years since the populations originally split apart - and in relation to our biological mechanisms, the differences ...

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by AstraSequi
Fri Feb 03, 2012 12:02 am
 
Forum: Evolution
Topic: Human evaluation
Replies: 24
Views: 6085
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