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Splicing

splicing

(Science: molecular biology) The process by which introns are removed from hnRNA to produce mature messenger RNA that contains only exons. Alternative splicing seems to occur in many proteins and by alternative exon usage a set of related proteins can be generated from one gene, often in a tissue or developmental stage specific manner.


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Re: Help with sequencing DNA.

... All sequences are written in standard 5' -> 3' direction. The reason I write "likely" is that there might be a pre-mRNA splicing event within the sequence that produces the mature mRNA strand. If so, the DNA sequence would be interrupted by an intron. If there is no splicing ...

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by jonmoulton
Mon Jun 29, 2009 4:47 pm
 
Forum: Molecular Biology
Topic: Help with sequencing DNA.
Replies: 3
Views: 189

Re: Exonic splicing enhancer

Wonderful. If I can assist with oligo design, let me know.

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by jonmoulton
Thu May 07, 2009 4:40 pm
 
Forum: Genetics
Topic: Exonic splicing enhancer
Replies: 3
Views: 369

Exonic splicing enhancer

Lay a steric-blocking oligo across the SNP and see if splicing changes.

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by jonmoulton
Wed May 06, 2009 3:24 pm
 
Forum: Genetics
Topic: Exonic splicing enhancer
Replies: 3
Views: 369

Exonic splicing enhancer

Hi everybody! How can I know if an identifyed SNP is present in a exonic splicing enhancer region? Thank you in advance ;)

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by Cscientist
Wed May 06, 2009 8:05 am
 
Forum: Genetics
Topic: Exonic splicing enhancer
Replies: 3
Views: 369

Re: Creating Cell Lines

Perhaps Splicing

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by CXCR4
Thu Apr 23, 2009 6:59 pm
 
Forum: Cell Biology
Topic: Creating Cell Lines
Replies: 3
Views: 372
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