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Splicing

splicing

(Science: molecular biology) The process by which introns are removed from hnRNA to produce mature messenger RNA that contains only exons. Alternative splicing seems to occur in many proteins and by alternative exon usage a set of related proteins can be generated from one gene, often in a tissue or developmental stage specific manner.


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Re: qPCR vs Northern Blot

I see that I reversed the sense of the observations - the Northern signal was maintained, the PRC signal decreased. A splicing change could still produce that result, as could the RNA degradation Jack mentioned.

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by jonmoulton
Mon Nov 11, 2013 4:38 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2783

Re: qPCR vs Northern Blot

... by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be spliced out of the pre-mRNA while the binding sites of the PCR primers remain. ...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2783

Use of cDNA when studying splicing

Hi I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples ...

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by nukeman
Wed May 15, 2013 11:33 am
 
Forum: Molecular Biology
Topic: Use of cDNA when studying splicing
Replies: 1
Views: 837

Re: Muscular dystrophy inheritance question

... downstream of the AAAAAAA the sequence is spliced to different sequences depending on the allele? Why, though, would the male show alternative splicing if he is homozygous? I've clearly not worked out this problem correctly. As you learn more about this one, please post it here.

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by jonmoulton
Thu Jan 10, 2013 3:59 pm
 
Forum: Molecular Biology
Topic: Muscular dystrophy inheritance question
Replies: 7
Views: 5683

Re:

... result (despite the exact same procedure and minimal scope for human error). My supervisor suggested that this could be something to do with the splicing nature of the gene.... he suggested that we stop trying to clone the whole gene and look at the splicing nature of ligase 6 before we re-attempt ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3386
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