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Spliceosome

spliceosome

(Science: molecular biology) A complex of small nuclear organelles in which the splicing and excision reactions that remove introns from precursor messenger RNA molecules occur.

One component of a spliceosome are small nuclear RNA molecules (u1, u2, u4, u5, u6) that collaborate with proteins, to help create rNA structures (snurps) that participate in hnRNA splicing.

Origin: Gr. Soma = body


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Idea to determine order of assembly of spliceosome

Isn't it possible to precipitate samples during different times? Like I had planned initially. If the spliceosome assembles stepwise, then wouldn't we be able to detect different masses at different points in time?

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by nukeman
Wed May 30, 2012 10:17 am
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7818

Idea to determine order of assembly of spliceosome

... either specific or unspecific NA probes will show you which proteins bind to nucleic acids. The question is, how to discriminate those bound in spliceosome from others. One way could be specific binding to introns (although that could show proteins bound to gDNA), but with that you could have ...

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by JackBean
Wed May 30, 2012 8:06 am
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7818

Re: Idea to determine order of assembly of spliceosome

How would I differentiate my target proteins from the rest of the proteins? Do you mean that I should use DNA/RNA that matches the pre-mRNA and that way isolate the target pre-mRNA along with the associated snRNP:s?

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by nukeman
Tue May 29, 2012 4:44 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7818

Re: Idea to determine order of assembly of spliceosome

The thing is that the questions are made so that we will pretend that we don't know that much about the spliceosome as we actually do, and from there proceed to map its function and so on. I should have written this more clearly. In essence, that means I "don't know" ...

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by nukeman
Tue May 29, 2012 12:29 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7818

Idea to determine order of assembly of spliceosome

... to present a suggestion of a series of methids to use in order to determine the order of binding of snRNP:s which eventually assemble to a spliceosome. My initial thought was to use in vitro splicing assay with a couple of extra steps. 1. Ligate a traget gene to a vector with some kind ...

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by nukeman
Mon May 28, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7818
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