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Spectrophotometer

Spectrophotometer

instrument used to determine the intensity of light of a wavelength transmitted by a solution. Provides measuring the amount of material in the solution.


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Re: blank and substrate control give OD reads

Is there anything in the buffer that absorbs light at the wavelength you are using for your OD readings? It might be worth running the buffer in a spectrophotometer and comparing against water at wavelengths near your OD measurement. Dear jonmoulton, thanks for your reply. >>>my buffer ...

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by intandiana
Sat Jan 18, 2014 8:39 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3146

Re: blank and substrate control give OD reads

Is there anything in the buffer that absorbs light at the wavelength you are using for your OD readings? It might be worth running the buffer in a spectrophotometer and comparing against water at wavelengths near your OD measurement.

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by jonmoulton
Thu Jan 16, 2014 3:23 pm
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 3146

Western Blot

mass spectrophotometer

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by JackBean
Sat Feb 16, 2013 9:57 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3201

Glucose concentration in plants

maybe you could use fehling's solution and measure color change using a spectrophotometer? There may or may not be conversions for the measurement you get; you'd have to look it up. Then you could look up the ratio of glucose to monosaccharides for the plant ...

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by plasmodesmata11
Wed Oct 31, 2012 3:01 am
 
Forum: Botany Discussion
Topic: Glucose concentration in plants
Replies: 2
Views: 1732

solutions containing different concentrations of oxaloacetat

... Dilution of 1:10 OAA: 900 μL water + 100 μL OAA malate: 900 μL water + 100 μL malate NADH: 900 μL water + 100 μL NADH we set the wavelength of the spectrophotometer to 340 nm. used a cuvette with 1 mL water as black, and them measured the absorbance of each of the dilutions of OAA, malate, or NADH, ...

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by ladwer
Sun Oct 07, 2012 2:38 am
 
Forum: General Discussion
Topic: solutions containing different concentrations of oxaloacetat
Replies: 1
Views: 1701
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