Spectrophotometer

simply by using uv spectrophotometer you can estimate the concentration of dna after pcr amplification

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... either method semi-quantitatively just by eye. If you are going to do it quantitatively, it would help to have access to either a colorimeter or spectrophotometer. Here is one way that does takes both approaches semi-quantitatively: http://www.biotopics.co.uk/nutrition/amylex.html There are a ...

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... a calculations based question, that i am having trouble with. the problem is i am trying to do a CS thermal aggregation assay on a fluorescence spectrophotometer and i cant seem to get the same light scattering units (not as high) as previous papers have so i presume my CS concentration is wrong.please ...

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... gel - gel is empty). Our primers: Pentamers: AATGC, CCTGA, GGTCA, AAGCC, TGACT Hexamers: AATGCA, TGCAAT, ATATGC, ATCATG We checked them using spectrophotometer – overything is OK. Finally, we use 2 ul of primer (2 x 100 pmol) according to article we found about doing PCR with hexamers (they ...

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... This is probably why you cannot see them in an EtBr gel. If you really think that you might not have any nucleotides in there, you could use a spectrophotometer to check the concentration. You need to run a special gel for mRNA. It needs to be a denaturing (formaldehyde) gel and it needs to ...

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