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Specific activity

specific activity

1. (Science: chemistry) The number of activity units (whatever is appropriate) per unit of mass, volume or molarity.

2. (Science: radiobiology) total activity of a given nuclide per gram of a compound, element or radioactive nuclide. Perhaps most often usage, in the context of radiochemicals, the number of microcuries per micromole.

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Mysterious Energy

Mental experience is determined by brain activity. Sensory experiences are only unique in that those brain events are linked ... white numbers are placed in front of a person with synesthesia, they can spot a specific number more quickly than the control. https://en.wikipedia.org/wiki/Synesthesia

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by wildfunguy
Tue Oct 29, 2013 2:36 pm
Forum: General Discussion
Topic: Mysterious Energy
Replies: 5
Views: 2486

low levels of protein but high activity levels

No, nobody. Now seriously. Do you know what is the specific activity of your pure enzyme? That could tell you something. How do you determine the protein level?

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by JackBean
Thu Nov 08, 2012 7:15 pm
Forum: Molecular Biology
Topic: low levels of protein but high activity levels
Replies: 3
Views: 1088

Multiple reactions buffer and enzymatic special activity

... this assignment: an enzyme has is provided in a concentration of mg/ml, activity mU/ml and specific activity of pmol/min/mg that is the one unit required to give pmol of product per ...

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by dalin
Wed Oct 17, 2012 3:40 am
Forum: Molecular Biology
Topic: Multiple reactions buffer and enzymatic special activity
Replies: 7
Views: 2955

Mastermix in PCR

... MgCl2 is a required cofactor for the Taq Polymerase to work. The activity of the Taq polymerase is sensitive to the concentration of MgCl2. ... DNA samples. However, this increases the amplification of "non-specific" PCR products. PCR products that are formed from the stabilization ...

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by daddyjames
Sun Oct 14, 2012 3:43 pm
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 3362

Relationship between total protein and enzyme activity

... for determining total protein (Bradford reagent) and enzymatic activity (GABA Assay). Here's my finding: total protein increased proportionately with fungal cell concentration. However, total activity and specific activity of GAD enzyme decreased from 30% w/v fungal cell concentration. ...

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by schroddycat
Sun Sep 02, 2012 12:34 pm
Forum: Molecular Biology
Topic: Relationship between total protein and enzyme activity
Replies: 1
Views: 4835
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