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Specific absorbance

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Re: Difference between spectrocopy

... a spectrum of light, either by: (1) setting the emission detector to a specific wavelength (actually a narrow passband) and scanning though a spectrum ... is collected by a detector. There are simpler instruments for measuring absorbance (colorimeters) which use fixed-wavelength filters instead ...

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by jonmoulton
Tue Nov 17, 2009 6:38 pm
Forum: General Discussion
Topic: Difference between spectrocopy
Replies: 3
Views: 7060

Re: absorbance

You can use absorbance at a specific wavelength to measure concentration in a simple solution. Spectra can be used to help identify the identity ...

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by jonmoulton
Tue Feb 24, 2009 5:46 pm
Forum: Molecular Biology
Topic: absorbance
Replies: 1
Views: 1055

gel electrophoresis & assays help please

... of alginate lyase by recombinants will be quantified by measurement of specific enzyme activity i am having 2 problems... I have 2 gels from electrophoresis. ... assays, we did a lyase assay and a protein assay. i have the levels of absorbance for each but i dot knwo what esle i need.... someone ...

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by RachBio
Fri Feb 06, 2009 11:32 pm
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 8539

Combining extinction coefficients?

To be more specific, the system of equations for a two-component system is: A1 = C1*E11 + C2*E21 A2 = C1*E12 + C2*E22 where A's are the absorbance at wavelenghts 1 or 2 E11 = extinction coefficient of component 1 at wavelength 1 E21 = extinction ...

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by blcr11
Wed Sep 19, 2007 4:47 pm
Forum: Molecular Biology
Topic: Combining extinction coefficients?
Replies: 4
Views: 11541

beta lactamase

... by chromatography. I can’t say for sure, since I don’t know the specifics of the lactamase you’re trying to purify, but I don’t think Q-sepharose ... the crude extract and wash with no/low salt buffer until the background absorbance at 280 nm is back to baseline indicating that all the non-binding ...

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by blcr11
Sat Mar 31, 2007 8:23 am
Forum: Microbiology
Topic: beta lactamase
Replies: 2
Views: 2646
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