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Southern blotting

Southern blotting --> blotting

(Science: molecular biology, procedure) general term for the transfer of protein, rNA or dNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called northern blots, DNA blots, southern, protein blots, western.


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Re:

... could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4). It ...

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by canalon
Sat May 14, 2011 3:02 am
 
Forum: Molecular Biology
Topic: History of Polymerase Chain Reaction
Replies: 5
Views: 2956

History of Polymerase Chain Reaction

... could be analyzed by restriction enzyme digestion, electrophoresis, southern transfer and hybridization to a cloned gene or oligonucleotide probes (3). However, southern blotting permitted only rudimentary mapping of genes in unrelated individuals (4).

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by angel92
Fri May 13, 2011 2:40 pm
 
Forum: Molecular Biology
Topic: History of Polymerase Chain Reaction
Replies: 5
Views: 2956

Devising Experiments to Determine Positive Regulation

... the circumstances in B and C? By experiment I mean, EMSA, Northern / Southern Blotting, DNase Sensitivity assay etc. Any ideas?

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by niravana21
Mon Mar 07, 2011 5:49 am
 
Forum: Cell Biology
Topic: Devising Experiments to Determine Positive Regulation
Replies: 1
Views: 775

DNA Profiling

... It could be just as straight dorward as PCR, Electrophoresis and southern blotting, but i want to be sure. If anyone has any idea i would be very grateful if you could ...

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by GeorgiaGS
Wed May 05, 2010 7:31 pm
 
Forum: Molecular Biology
Topic: DNA Profiling
Replies: 1
Views: 3083

PCR to screen for diseases

... the DNA, how would you screen for the genetic disorders if not via southern blotting? Thanks

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by james86
Sun Mar 21, 2010 1:45 pm
 
Forum: Genetics
Topic: PCR to screen for diseases
Replies: 15
Views: 4943
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