Southern blots

... fragments sizes of 3.7, 6.3, 7.0, 8.8 and 9.6 kb. Autoradiograph from southern hybridization showed that fragments 7.0 and 9.6 are radioactive, ... HindIII sites. b) A radio-labeled gene x probe is added to the Southern blots that contain the DNA. Resulting autoradiograph showed hybridisation ...

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... probe prepared by labeling the original gene is used to analyze southern blots of three different restriction digest.prepare a map of the insert region containing the ...

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... are both methods for looking at sizes of particular polynucleotides. Southern blots are for DNA while Northern blots are for RNA. The methods are basically the same, though ...

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... to avoid getting polymerizing regions against the glass. I was doing Southern blots; I don't know how important it is for normal check gels and whatnot, but when casting, ...

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... and so on) you can analyse the size of the fragments of the separate blots and combine this to get the restriction map. First you only use restriction enzyme 1 and do a gel electrophoresis and Southern blot and analyse the length of the fragments (complete digest) Then repeat ...

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