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Dictionary » S » Southern blot

Southern blot

southern blot

A technique used for searching for a specific dNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix dNA fragments

5. probe with labelled messenger RNA or cDNA

6. wash

complementary mRNA/cDNA fragments will have hybridised.


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molecular biology

... The following gel diagram shows the pattern of bands observed in a Southern blot for three tightly linked SSR molecular markers in and flanking the p16 gene in a family ...

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by soniasunny111
Sat Aug 20, 2011 10:45 am
 
Forum: General Discussion
Topic: molecular biology
Replies: 1
Views: 2288

finding out if resistance is from mutation or hybridization?

... gives the resistance), that isn't in the wildtype. Could a Southern blot be used to test for this microsat? If so, how would we know if it was weedy rices own ...

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by AE35
Sat Jun 11, 2011 12:48 am
 
Forum: Genetics
Topic: finding out if resistance is from mutation or hybridization?
Replies: 4
Views: 2144

nuclear acid probe labeling

... manual that is available was generally a very good information for all Southern and Northern blot related thing (even if you use other products)

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by canalon
Sat Sep 25, 2010 6:16 pm
 
Forum: Molecular Biology
Topic: nuclear acid probe labeling
Replies: 3
Views: 2456

Re:

... cases. Okay So I could just cut one sequence with ECO R1 and then do a southern blot hybridization. I understand the concept of cutting. But I don't understand the concept ...

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by doctobe
Sat Feb 20, 2010 6:31 am
 
Forum: Molecular Biology
Topic: DNA sequenced. Erased labels. Now re-identify.
Replies: 12
Views: 3481

DNA sequenced. Erased labels. Now re-identify.

... it. Tsp for the B strand and Eco R1 for the A strand. Then do a southern blot hybridization since it I believe it is a gDNA I believe. I pmed you for more detail and ...

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by doctobe
Tue Feb 16, 2010 1:47 am
 
Forum: Molecular Biology
Topic: DNA sequenced. Erased labels. Now re-identify.
Replies: 12
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