Southern blot

southern blot

A technique used for searching for a specific dNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix dNA fragments

5. probe with labelled messenger RNA or cDNA

6. wash

complementary mRNA/cDNA fragments will have hybridised.


Please contribute to this project, if you have more information about this term feel free to edit this page



This page was last modified on 3 October 2005, at 21:16. This page has been accessed 2,170 times. 
What links here | Related changes | Permanent link