Southern

... chose a vector with SacB for marker-free knockout. The recombinants are positive for PCR detection. But all of the recombinants are incorrect for southern blotting, the gene still exist in genome. I think non-homologous recombination happened. Anybody knows what can I do? @-@

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sequence, microarray, Southern blot

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... Ecor 1 and the plasmid with the insert selected,grown up and plasmid isolated.A probe prepared by labeling the original gene is used to analyze southern blots of three different restriction digest.prepare a map of the insert region containing the gene and explain southern blot data? Asp 1-----3.6,2.4,2.1,.9 ...

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... the hourglass markings underneath her abdomen." "For the Northern Black Widow, the hourglass is distinct, but is broken(1) (whereas the Southern Black Widow's hourglass is complete) and typically there is a row of red spots down the middle of the back and possibly some diagonal whitish ...

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... 2. Digest large amount with the enzyme (6 cutter preferred) that does NOT cut your gene overnight. 3. Run a long gel (5-6HR at 30V). 4. Perform southern blot. 5. Analyze results: 1 band = 1 locus, etc... Hope this helps.

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