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Dictionary » S » Southern Southern![]()
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Results from our forumhow to avoid Non-homologous recombination in gene knock-out... chose a vector with SacB for marker-free knockout. The recombinants are positive for PCR detection. But all of the recombinants are incorrect for southern blotting, the gene still exist in genome. I think non-homologous recombination happened. Anybody knows what can I do? @-@
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Re: Restricion map.... Ecor 1 and the plasmid with the insert selected,grown up and plasmid isolated.A probe prepared by labeling the original gene is used to analyze southern blots of three different restriction digest.prepare a map of the insert region containing the gene and explain southern blot data? Asp 1-----3.6,2.4,2.1,.9 ...
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Re: Black shiny spider in Northern KY... the hourglass markings underneath her abdomen." "For the Northern Black Widow, the hourglass is distinct, but is broken(1) (whereas the Southern Black Widow's hourglass is complete) and typically there is a row of red spots down the middle of the back and possibly some diagonal whitish ...
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How to find out if a gene was present in more than 1 locus?... 2. Digest large amount with the enzyme (6 cutter preferred) that does NOT cut your gene overnight. 3. Run a long gel (5-6HR at 30V). 4. Perform southern blot. 5. Analyze results: 1 band = 1 locus, etc... Hope this helps.
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