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Solvent extraction

solvent extraction

A method of separation used to purify vegetable oils.

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Re: RNA prep help

... extracting RNA from it's native source and using it. If it's a tissue extraction I tend to get the tissue the same day as whatever you need the ... often result in some kind of salt crystal that would go into the solvent, and i suspected that could have affected column binding conditions. ...

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by vastgenome
Mon Apr 12, 2010 5:51 am
Forum: Molecular Biology
Topic: RNA prep help
Replies: 4
Views: 3682

Isolating algae pigments - Paper chromatography?

... as a high school student). You would probably need to use some organic solvent, as chlorophyll isn't water soluble. Benzen, acetone etc are good. ... Here is a link http://www.monsanto.com/pdf/responsibility/chlorophyll_extraction.pdf to a protocol describing chlorophyll extraction using isopropanol. ...

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by MrMistery
Tue Sep 01, 2009 1:40 pm
Forum: Botany Discussion
Topic: Isolating algae pigments - Paper chromatography?
Replies: 1
Views: 4348

Beet Cell Damage

... to denature proteins and disrupt membrane integrity. Second, chloroform extraction will separate the lipids from the membrane leaving the proteins ... proteins tend to aggregate, as their hydrophobic regions try to exclude solvent by associating with other hydrophobic regions on anything that happens ...

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by blcr11
Tue Dec 09, 2008 2:09 pm
Forum: Cell Biology
Topic: Beet Cell Damage
Replies: 2
Views: 5998

The Fiber Disease

... chemicals. Benzene and ether have been used, but a popular chemical for solvent extraction is hexane, which is relatively inexpensive. The downside to using solvents for oil ...

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by Skytroll
Thu Nov 16, 2006 7:06 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6246191

The Fiber Disease

... analysis of pesticides in food. Development and evaluation of the “solvent in silicone tube extraction” (SiSTEx) approach. Effective and efficient analytical methods are needed to detect ...

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by Nadas Moksha
Sun Nov 05, 2006 3:33 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6246191

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