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(Science: abbreviation) solution.

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Preparing buffer solns.

I have an assignment to prepare certain buffer solns from certain chemicals and I'm struggling with it, so some help would be greatly appreciated. I've looked in my old chem books to try to find guidance, but unsuccessfully. Anyway, here's an example ...

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by sunnidaze
Sat Sep 03, 2011 6:47 pm
Forum: Molecular Biology
Topic: Preparing buffer solns.
Replies: 6
Views: 3258

some general capsid questions

What kind of proteins are the capsid shells made of? Will a collection of capsid proteins in soln' generally form an icosahedral (or other geometric shape, depending on protein..) shape; Will they from encapsulating shapes on their own? can capsid shells be manufactured?

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by raneynickel
Sat Aug 08, 2009 12:50 am
Forum: Microbiology
Topic: some general capsid questions
Replies: 4
Views: 2150

Apoferritin characterictic pKa?

... the pKa value for apoferritin, or where could I find out> I am trying to understand why my supervisor has decided to use NaAcetate as a buffer soln in a protein crystallizing expt. I am doing. Is the crystallizing process of apoferritin a proton releaser or opposite? Cheers!

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by philip041
Wed Jan 14, 2009 4:31 pm
Forum: Molecular Biology
Topic: Apoferritin characterictic pKa?
Replies: 0
Views: 692

Citrate synthase stock calculation

... units (not as high) as previous papers have so i presume my CS concentration is wrong.please help as im really at the end of my tether!! my stock soln of CS 7.17mg/ml MW of CS 85,000(although some seem to work with monomer value of 42,500) protocol calls for me to make a 65uM stock of CS So in ...

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by proteinz
Tue Oct 07, 2008 10:37 am
Forum: Molecular Biology
Topic: Citrate synthase stock calculation
Replies: 1
Views: 1417

Quantity of ethidium bromide

I think I add about.... 6microliters of EtBR to each 100mL of agarose soln. (I add it when the agarose soln. is 'cool' enough to pour) .. however, i do not know the concentration of the EtBR off hand.... but I find that a little goes a long way.

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by LilKim
Sun Apr 22, 2007 1:17 am
Forum: Molecular Biology
Topic: Quantity of ethidium bromide
Replies: 2
Views: 1069
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