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Soft agar

soft agar

(Science: cell culture) Semi solid agar used to gelate medium for culture of animal cells. Placed in such a medium, over a denser agar layer, the cells are denied access to a solid substratum on which to spread, so that only anchorage independent (usually transformed) cells are able to grow.


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Swarming Assay

Hi, I have a question considering a swarming assay on E.coli... I did a soft agar (0.3%), in LB, and placed a drop of bacteria in the middle of a plate. But after 24h, ...

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by SpellForce
Wed Oct 29, 2014 8:21 pm
 
Forum: Microbiology
Topic: Swarming Assay
Replies: 0
Views: 459

Need URGENT help identifying unkown bacteria!

... is identify an unknown bacteria. We inoculated 3 plates a Nutritional Agar plate, Blood Agar, and endo Agar. The morphology was convex, more circular(mucoid ... was still liquid, but it was very thick. Acid ? Alkaline ? Hard curd - Soft curd ? I think it may have been a rennet curd Reduction + ...

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by graymonkey44
Tue Aug 02, 2011 1:35 pm
 
Forum: Microbiology
Topic: Need URGENT help identifying unkown bacteria!
Replies: 1
Views: 6266

anyone work with SeaPlaque Agarose

I used the SeaPlaque Agarose from Takara to do the soft agar assay. Base agar is 0.6% and top agar is 0.3%. The problem is that my gel always melted. I created ...

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by huyenkobe
Sat Feb 19, 2011 2:23 am
 
Forum: General Discussion
Topic: anyone work with SeaPlaque Agarose
Replies: 0
Views: 1121

Plaque assay

... say about your phage culture? What is the difference between Hard and soft agar? I don't know your protocol, but try to think what would have happened if you had followed ...

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by canalon
Thu Dec 14, 2006 1:33 pm
 
Forum: Microbiology
Topic: Plaque assay
Replies: 1
Views: 4090

Plaque assay

... Also....a question reads " why was it important to use a hard agar with a soft agar overlay instead of vice versa???" Thank you

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by csumicroa
Thu Dec 14, 2006 2:43 am
 
Forum: Microbiology
Topic: Plaque assay
Replies: 1
Views: 4090
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