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Soft agar

soft agar

(Science: cell culture) Semi solid agar used to gelate medium for culture of animal cells. Placed in such a medium, over a denser agar layer, the cells are denied access to a solid substratum on which to spread, so that only anchorage independent (usually transformed) cells are able to grow.


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Need URGENT help identifying unkown bacteria!

... is identify an unknown bacteria. We inoculated 3 plates a Nutritional Agar plate, Blood Agar, and endo Agar. The morphology was convex, more circular(mucoid ... was still liquid, but it was very thick. Acid ? Alkaline ? Hard curd - Soft curd ? I think it may have been a rennet curd Reduction + ...

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by graymonkey44
Tue Aug 02, 2011 1:35 pm
 
Forum: Microbiology
Topic: Need URGENT help identifying unkown bacteria!
Replies: 1
Views: 5670

anyone work with SeaPlaque Agarose

I used the SeaPlaque Agarose from Takara to do the soft agar assay. Base agar is 0.6% and top agar is 0.3%. The problem is that my gel always melted. I created ...

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by huyenkobe
Sat Feb 19, 2011 2:23 am
 
Forum: General Discussion
Topic: anyone work with SeaPlaque Agarose
Replies: 0
Views: 1010

Plaque assay

... say about your phage culture? What is the difference between Hard and soft agar? I don't know your protocol, but try to think what would have happened if you had followed ...

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by canalon
Thu Dec 14, 2006 1:33 pm
 
Forum: Microbiology
Topic: Plaque assay
Replies: 1
Views: 3867

Plaque assay

... Also....a question reads " why was it important to use a hard agar with a soft agar overlay instead of vice versa???" Thank you

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by csumicroa
Thu Dec 14, 2006 2:43 am
 
Forum: Microbiology
Topic: Plaque assay
Replies: 1
Views: 3867

The Fiber Disease

... as a periorbital cellulitis or mass with progressive local invasion of soft tissue (85, 94). The arterial form is the most serious and is largely ... material seeded onto mycologic medium, such as Sabouraud's glucose agar (56). Demonstration of biflagellate zoospores may be accomplished using ...

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by hartuk
Thu Nov 09, 2006 9:58 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5560672
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