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Snrnp

snRNP

(Science: molecular biology) small nuclear ribonucleoprotein.

See: small nuclear RNA.


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Idea to determine order of assembly of spliceosome

... samples at different points in time, how would I go on about analysing them? Is it feasible to use MS here or are the differences (when the next snRNP joins in) in snRNP mass enough for me to be able to detect change in mass on a western?

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by nukeman
Wed May 30, 2012 9:00 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7823

Re: Idea to determine order of assembly of spliceosome

... the proteins? Do you mean that I should use DNA/RNA that matches the pre-mRNA and that way isolate the target pre-mRNA along with the associated snRNP:s?

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by nukeman
Tue May 29, 2012 4:44 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7823

Idea to determine order of assembly of spliceosome

... I've got this assignment where I'm supposed to present a suggestion of a series of methids to use in order to determine the order of binding of snRNP:s which eventually assemble to a spliceosome. My initial thought was to use in vitro splicing assay with a couple of extra steps. 1. Ligate a ...

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by nukeman
Mon May 28, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7823

Re: alternative splicing

Hi Noy K, Alternative splicing is regulated by a number of factors. One is the strength of the snRNP binding sites, the U1 and U2 sites of the splice donor and splice receptor of the major splicosome. Non-consensus splice sites bind snRNPs less strongly and so are less ...

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by jonmoulton
Fri Apr 18, 2008 3:34 pm
 
Forum: Molecular Biology
Topic: alternative splicing
Replies: 1
Views: 1716


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