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Smeared

smeared

(Science: zoology) Having the colour mark ings ill defined, as if rubbed; as, the smeared dagger moth (Apatela oblinita).


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Primers Tm

... 240 bp. I am using KOD-Plus kit. cycling conditions are 94/2 min 94/15 sec 55/ 30 sec 68/ 15 sec I got faint bands but at the same time it was smeared, I am using the optimal reaction volume from the kit and I tried it before with other genes so I dont think its MgSO4 or DNA template conc., ...

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by bravebeaker
Thu Oct 11, 2012 7:18 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 2951

Blood Cells. Help needed? :S

... place a cover slip on top to examine it. 2: Take a microscope slide, place a drop of blood on it, and smear it out by using a cover slip. Let the smeared blood air-dry for 10-15 seconds, then cover it with methanol and let the alcohol evaporate. Afterwards cover the area with Gimesa-stain and ...

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by boogienorway89
Thu Feb 17, 2011 6:21 pm
 
Forum: Cell Biology
Topic: Blood Cells. Help needed? :S
Replies: 5
Views: 3155

smear after restriction digestion

Hi everybody, Im getting problem with restriction digestion of plasmid DNA as Im getting smeared band, on digestion of plasmid with EcoRv....Plz help soon

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by jasen
Sat Feb 05, 2011 12:38 pm
 
Forum: Molecular Biology
Topic: smear after restriction digestion
Replies: 23
Views: 24944

Calculating the size of E. coli chromosome?

... restriction endonuclease digestion of DNA. the results after electrophoresis show the plasmid DNA in discrete bands but the chromosomal DNA is a smeared profile. The question is to design a variation of the experiment that will enable you to calculate the size of the E.coli chromosome given that ...

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by sheeshamagurlie
Mon Oct 11, 2010 2:15 pm
 
Forum: Molecular Biology
Topic: Calculating the size of E. coli chromosome?
Replies: 4
Views: 6643

TRUE postives or non specific bands, pls help

Use negative control from non-infected human. Well, than you should check out, what has changed between tests, where it was fine and where it was smeared ;)

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by JackBean
Sat Jan 09, 2010 1:41 pm
 
Forum: Molecular Biology
Topic: TRUE postives or non specific bands, pls help
Replies: 13
Views: 3610
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