Dictionary » S » Smear

Smear

smear

1. To overspread with anything unctuous, viscous, or adhesive; to daub; as, to smear anything with oil. Smear the sleepy grooms with blood.

2. To soil in any way; to contaminate; to pollute; to stain morally; as, to be smeared with infamy.

Origin: OE. Smeren, smerien, AS. Smierwan, smyrwan, fr. Smeoru fat, grease; akin to D. Smeren, OHG. Smirwen, G. Schmieren, Icel. Smyrja to anoint. See Smear.


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western blotting of DNA-binding protein of mammalian cell

... pellet 50ul of 2X sample buffer added, boiled for 5 min in 95C and SDS-PAGE... duing SDS-PAGE in not getting a banding pattern instead i got full smear of gel....simultaneously i have added other sample they ran properly but not this cell lysate... what could be problem..how to get a clear SDS-PAGE ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 424

pfge problem H.influenzae

I am doing PFGE with Haemophilus influenzae. Also i am using SmaI restriction enzyme, but i get this profile. Not as smear, actually i am counting many bands but as we know we have to find minumum 8 bands. Any idea about band profile ? Is this a good profile? Thank you

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by biologistesab
Sun Nov 09, 2014 11:14 am
 
Forum: Molecular Biology
Topic: pfge problem H.influenzae
Replies: 0
Views: 494

asseveration at the annihilation of unalloyed's shuck cohere

... the task relationship wrongfully, on the side of the benefit of standard, at without to hunt down contractual disciplinary procedures or by smear to furnish odbojnice przemyslowe sierpc justified pay impaired the contract, then the covenants longing automatically suit unenforceable, whether ...

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by Hellsinggareeg
Mon Aug 04, 2014 6:39 am
 
Forum: Microbiology
Topic: Detect influenza in saliva
Replies: 14
Views: 22666

An interesting PCR smearing...I dont know what to do :(

Just a stupid idea, but if you digest your DNA with some RE cutting between your primers, do you get rid of the smear?

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by JackBean
Sat Jun 28, 2014 6:08 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8810

Re: An interesting PCR smearing...I dont know what to do :(

... for amplifying all 3 exons . Interestingly sometimes I do get a single bright band , but this happens for 1 in 10 samples and rest appear as smear . I checked the DNA quality and its good ,,..Really I dont know what to do next .. plz suggest. Thanks.

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8810
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