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Site preparation

site preparation

Various treatments applied to a harvested area to promote regeneration of the site.


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Re: How can a protein without a G-site bind to GSH?

... is the result i got. I was hoping that this protein would be a new protein. I just wanted assurance to rule out the possibility of this being a preparation error. Thank you for your input.

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by hurzz
Mon Nov 26, 2012 3:40 am
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3286

MALDI; fusion protein cleaving from a peptide

... peptide (3,2 kDa) from a fusion protein.The construct contains protease site between fusion protein and the peptide of interest (actually I have ... interrupt MALDI? Or may you share some experience about tricine gel preparation which could help me? P.S. There is no problem with MALDI apparatus ...

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by Valya
Wed Mar 28, 2012 8:42 pm
 
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 2031

Electrophoresis of a plasmid

... the plasmid could have in a gel three bans depending of the quality of preparation. Upper is supelcoil band, medium linear plasmid (broken in two ... or one. Different case is if you digest it with a RE wich have only a site of cut you will see only a band,: linear digested , if you cut at same ...

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by protold
Thu May 05, 2011 5:28 am
 
Forum: Cell Biology
Topic: Electrophoresis of a plasmid
Replies: 2
Views: 4103

Biology - Preparation of Solutions

Hey peoples! Came across this site and thought it would be a good idea to join :) Now I’ve got a test tomorrow haha yep studying last minute practically... anyway the revision questions we got out teacher hasn’t even explained to us how ...

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by Horizon
Mon Apr 26, 2010 10:13 am
 
Forum: General Discussion
Topic: Biology - Preparation of Solutions
Replies: 1
Views: 847

spectrum of riboflavin

... protein --calculate the dna conc. of the standard calf thymus dna preparation and te %of protein present.. so is it says calculate shouldnt ... do I use (@280nm) c=extinction coefficient (the standard one from the site I see it's 1?-is this right?)*OD (which is the absobance I take it) ...

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by biology_06er
Sat Apr 14, 2007 11:07 pm
 
Forum: Cell Biology
Topic: spectrum of riboflavin
Replies: 5
Views: 6937
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