Site preparation

... is the result i got. I was hoping that this protein would be a new protein. I just wanted assurance to rule out the possibility of this being a preparation error. Thank you for your input.

See entire post

... peptide (3,2 kDa) from a fusion protein.The construct contains protease site between fusion protein and the peptide of interest (actually I have ... interrupt MALDI? Or may you share some experience about tricine gel preparation which could help me? P.S. There is no problem with MALDI apparatus ...

See entire post

... the plasmid could have in a gel three bans depending of the quality of preparation. Upper is supelcoil band, medium linear plasmid (broken in two ... or one. Different case is if you digest it with a RE wich have only a site of cut you will see only a band,: linear digested , if you cut at same ...

See entire post

Hey peoples! Came across this site and thought it would be a good idea to join :) Now I’ve got a test tomorrow haha yep studying last minute practically... anyway the revision questions we got out teacher hasn’t even explained to us how ...

See entire post

... protein --calculate the dna conc. of the standard calf thymus dna preparation and te %of protein present.. so is it says calculate shouldnt ... do I use (@280nm) c=extinction coefficient (the standard one from the site I see it's 1?-is this right?)*OD (which is the absobance I take it) ...

See entire post