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Gene knockdowns in the wasp Nasonia vitripennis

... significant knockdown of later-acting genes. To overcome this limitation, we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon-intron junction in the homeobox. These two independent morpholinos are expected to disrupt Nv eve activity ...

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by jonmoulton
Fri Mar 07, 2014 5:57 pm
 
Forum: Molecular Biology
Topic: Gene knockdowns in the wasp Nasonia vitripennis
Replies: 0
Views: 57

where can I learn synthetic biology?

... it in Wikipedia, learn its history and usage, available programming languages and then search for ebooks, join and post in forums/Q&A style sites and subscribe to few newsletters for news in the field. With synth bio, I've only been able to find books that talk about particular subject (no ...

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by cipher0
Sun Feb 09, 2014 12:28 pm
 
Forum: General Discussion
Topic: where can I learn synthetic biology?
Replies: 0
Views: 368

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 15561

Re: Re:

... and not a lot is known about them. There are lots of people suffering from Bartonella who don't get much help and hang around on one or two sites on the net to get information and identify the handful of doctors who treat it. Some have got better with long-term abx. I am 6 months into abx ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3427

Re: Re:

... any pathogens in the blood tend to stand up in the tests. With the skin-prick method I'd say applying 70% ethanol carefully around the puncture site may help, but more important is to puncture the skin a neatly as possible (no scratching), let a drop or two of blood to flow from the puncture ...

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by biohazard
Fri Dec 13, 2013 8:13 am
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 3427
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