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Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is ...

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OK, so you have DNA sequences from related species which are somewhere diverse, somewhere are identical. For example the region coding for active site will be probably conserved, while some loops on surface of the protein will be divergent. Both on DNA and amino acid level. You can design primers ...

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... members of this community group. I am a newbie here. I am very interested to join with the discussion board and to share my views. I came to your site with a lot of searches. If any type of inconvenience, then I apologize for that. Best Regards, Alex Johns :)

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... the male is a homozygote and the female is heterozygous (at least over the sequence given in the lower traces). The female might have a splice site mutation, as the sequence across the polymorphism is either ATGTGAAG or ATGGGAAG and a GT, present in only one allele, is the canonical start of ...

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... better reason for me or anyone to spend unnecessary money to get the high purity Triacontanol. I really hope there are true experts here on this site who have had actually used and tested with low purity Triacontanol and are able to provide such invaluable knowledge to those who seek to save ...

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