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Single-blind method

single-blind method

A method in which either the observer(s) or the subject(s) is kept ignorant of the group to which the subjects are assigned.

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amplification in no RT control

... following exposure to antimicrobials. RNA has been isolated by trizol method and subsequently 'cleaned' on column and finally been DNase treated ... this is occurring ~20 cycles later than RT+ samples. I am getting single peak melt curves and my NTC is not amplifying. This suggests to me ...

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by biomart
Fri Mar 20, 2015 1:58 am
Forum: General Discussion
Topic: amplification in no RT control
Replies: 1
Views: 49

An Overview of Embryonic Stem Cells

... initially created for reproductive, purposes. There is an alternative method for deriving human ES cells, namely, the somatic nuclear transfer, ... flat rather than spherical colonies, also more easily dissociated into single cells than their mouse counterparts. Human ES cells are also unresponsive ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
Forum: Cell Biology
Topic: An Overview of Embryonic Stem Cells
Replies: 1
Views: 580

Re: Re:

... in the blood tend to stand up in the tests. With the skin-prick method I'd say applying 70% ethanol carefully around the puncture site may ... know which photos are from which slides - they could all be from a single slide for all i know, though I'd have thought my vet friend would ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 15887

Problems making chemically competent cells

... is cold. Any help here would be appreciated. I've pasted the exact method I use below this: • Pre-chill a flask on ice for the chilling step ... eppendorf rack in the -80) 1. Make a 5ml pre-culture overnight from a single colony, in SOC medium 2. The next day, inoculate 100ml pre-warmed ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 3642

Re: Markerless mutants using suicide vector

... than other gram-negative bacteria. We had been able to generate a single mutant using this method before, but we're having trouble deleting a gene within the same region (operon), and ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 3620
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