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Sin recombinase

sin recombinase

(Science: enzyme) From staphylococcus aureus; genbank l23109; do not confuse with sin gene product, a repressor protein from bacillus subtilis

Registry number: EC 2.7.7.-

Synonym: sin gene product, staphylococcus


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gene knockout mice

Is it possible to get the striatum astrocyte-specific Cre recombinase–mediated knockout of XX gene knockout mice? Any one understand how to make the knockout locate in striatum?or any other good ideas to knockout one gene in one special cell from one ...

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by w19910411
Wed Mar 18, 2015 6:22 am
 
Forum: Genetics
Topic: gene knockout mice
Replies: 1
Views: 514

Knockout mice

... mice this morning. "Knockdown of Cdc42 in the developing retina was achieved by crossing Cdc42 floxed mice [29] with mice expressing cre recombinase under the control of the α element of the Pax6 promoter [30]. " From Mat'l & Methods: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553133/

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by jonmoulton
Wed Feb 06, 2013 4:24 pm
 
Forum: Genetics
Topic: Knockout mice
Replies: 5
Views: 5635

Alignment in Site-specific Recombination

... for my exams and have a problem about site-specific recombination: When recombination between two DNA molecoules with lox sites through Cre-recombinase occurs, those two lox sites first have to come close to each other for recombination to start. How is this achieved, how do they find each ...

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by Ebson
Wed Jul 14, 2010 10:08 am
 
Forum: Genetics
Topic: Alignment in Site-specific Recombination
Replies: 0
Views: 799

What is resolvase?

... In a Holliday junction, does it cause the crossing over or does it does it cut the strands after it crosses over? And is it the same thing as recombinase? Thanks!

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by BioGradStudent
Tue Oct 21, 2008 1:09 am
 
Forum: Genetics
Topic: What is resolvase?
Replies: 0
Views: 1467

Re: Allele symbols/acronyms?

... It stands for "floxed" which itself stands for "flanked by lox P sites" the lox P sequence being the recognition site for Cre recombinase. See Kuhn, et. al. (1995) Science 269, 1427-1429. You put Cre recombinase under the control of a promoter (Mx1) which is sensitive to stimulation ...

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by blcr11
Wed Sep 10, 2008 4:20 pm
 
Forum: Genetics
Topic: Allele symbols/acronyms?
Replies: 7
Views: 11016
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