Silver protein stain

... occasion on simple gel where I had too much protein (intense coomassie staining), the silver stain gave a blank center with only the side lightly colored. ...

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Hi everyone. I have a problem staining an second dimentional gel of proteins. I get negative spots after stain with silver nitrate. I had done different silver staining protocols and the ...

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Hello, I am not sure if the Coomasie staining interferes with the silver staining but it may be the case because both stains interact with the aminoacids of the protein. Additionally you need to fix the gel prior to the staining ...

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... in polyacrylamide gels. First gel with silver staining, second for Western blotting/for an one biological ... I give to film and I choose a place of presence my protein according to pI and Mr my protein. Feel free to ...

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... or anti-antibody conjugate. You could try silver staining, but that won’t be specific for your protein. You might try zymography if your enzyme has a substrate ...

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