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Silver protein stain

silver protein stain

(Science: technique) A silver proteinate complex used in staining nerve fibres, nerve endings, and flagellate protozoa; also used to demonstrate phagocytosis in living animals by the cells of the reticuloendothelial system.


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Silver staining problem

... occasion on simple gel where I had too much protein (intense coomassie staining), the silver stain gave a blank center with only the side lightly colored. ...

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by canalon
Thu Dec 09, 2010 6:58 pm
 
Forum: General Discussion
Topic: Silver staining problem
Replies: 2
Views: 1993

Silver staining problem

Hi everyone. I have a problem staining an second dimentional gel of proteins. I get negative spots after stain with silver nitrate. I had done different silver staining protocols and the ...

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by hericorreia
Thu Dec 09, 2010 10:13 am
 
Forum: General Discussion
Topic: Silver staining problem
Replies: 2
Views: 1993

Silver staining after coomassie blue

Hello, I am not sure if the Coomasie staining interferes with the silver staining but it may be the case because both stains interact with the aminoacids of the protein. Additionally you need to fix the gel prior to the staining ...

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by ggsteam
Sun Nov 22, 2009 6:54 pm
 
Forum: Molecular Biology
Topic: Silver staining after coomassie blue
Replies: 2
Views: 4360

Re: Matching 2D membrane spots to the gel

... in polyacrylamide gels. First gel with silver staining, second for Western blotting/for an one biological ... I give to film and I choose a place of presence my protein according to pI and Mr my protein. Feel free to ...

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by JackBean
Mon Sep 21, 2009 8:39 pm
 
Forum: Molecular Biology
Topic: Matching 2D membrane spots to the gel
Replies: 1
Views: 1719

SDS PAGE

... or anti-antibody conjugate. You could try silver staining, but that won’t be specific for your protein. You might try zymography if your enzyme has a substrate ...

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by blcr11
Fri Apr 20, 2007 11:34 am
 
Forum: General Discussion
Topic: SDS PAGE
Replies: 1
Views: 1298


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