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Silver-ammoniacal silver stain

silver-ammoniacal silver stain

(Science: technique) A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin.

Synonym: ag-AS stain.


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electrophoretic mobility shift assay (EMSA)

... you cannot see the blue front? It cannot just disappear. Did you try to stain your gel with silver/Coomassie?

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by JackBean
Mon Nov 11, 2013 1:50 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 704

Silver staining problem

... occasion on simple gel where I had too much protein (intense coomassie staining), the silver stain gave a blank center with only the side lightly colored. But I never had a negative ...

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by canalon
Thu Dec 09, 2010 6:58 pm
 
Forum: General Discussion
Topic: Silver staining problem
Replies: 2
Views: 1736

Silver staining problem

Hi everyone. I have a problem staining an second dimentional gel of proteins. I get negative spots after stain with silver nitrate. I had done different silver staining protocols and the result is always the same. negative ...

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by hericorreia
Thu Dec 09, 2010 10:13 am
 
Forum: General Discussion
Topic: Silver staining problem
Replies: 2
Views: 1736

Silver staining after coomassie blue

Hello, I am not sure if the Coomasie staining interferes with the silver staining but it may be the case because both stains interact with the aminoacids of the protein. ...

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by ggsteam
Sun Nov 22, 2009 6:54 pm
 
Forum: Molecular Biology
Topic: Silver staining after coomassie blue
Replies: 2
Views: 4052

Silver staining after coomassie blue

Hello, I stain my 2D gels with coomassie blue and sometimes about a week later, perform silver staining on them. I was wondering if the original coomassie blue staining has any effect on the quality ...

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by samkh918
Mon Nov 09, 2009 7:53 pm
 
Forum: Molecular Biology
Topic: Silver staining after coomassie blue
Replies: 2
Views: 4052
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