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Signal

signal

1. Noticeable; distinguished from what is ordinary; eminent; remarkable; memorable; as, a signal exploit; a signal service; a signal act of benevolence. As signal now in low, dejected state As erst in highest, behold him where he lies. (milton)

2. Of or pertaining to signals, or the use of signals in conveying information; as, a signal flag or officer. The signal service, a bureau of the government (in the united states connected with the war department) organised to collect from the whole country simultaneous raports of local meteorological conditions, upon comparison of which at the central office, predictions concerning the weather are telegraphed to various sections, where they are made known by signals publicly displayed. Signal station, the place where a signal is displayed; specifically, an observation office of the signal service.

Synonym: eminent, remarkable, memorable, extraordinary, notable, conspicuous.

Origin: From signal, n., cf. F. Signale.


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How can I add a kozak sequence in plasmid pMT/bip/V5-His A

... molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add ...

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by yiwi123
Tue Mar 12, 2013 8:04 pm
 
Forum: Molecular Biology
Topic: How can I add a kozak sequence in plasmid pMT/bip/V5-His A
Replies: 1
Views: 35

Two-color in situ help

Problem seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

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by vastgenome
Sat Mar 09, 2013 4:21 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 253

Why would the identical amino acids region of certain specie

... of only one species. On the other hand, you can design primers to the region which is the same in all species and then the primers will give signal from all species. You can use it for detection of one taxon, say you will want to know whether Klebsiella bacteria are present in your sample. ...

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by JackBean
Mon Feb 18, 2013 3:51 pm
 
Forum: Genetics
Topic: Why would the identical amino acids region of certain specie
Replies: 2
Views: 179

Re: Two-color in situ help

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by jonmoulton
Thu Feb 14, 2013 4:23 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 253

Re: nuclear DNA binding

... nail that down. You could also fluorescent-tag the protein and microinject it or otherwise transfect it into the cytoplasm, then watch where the signal goes. It would be interesting to see if it either accumulates in the nucleus or is excluded from the nucleus.

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by jonmoulton
Tue Jan 22, 2013 6:39 pm
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 297
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