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Shuttle vector



A vector that can replicate in more than one host organisms or two different cell types (e.g. a prokaryotic cell and a eukaryotic cell).


An example is the yeast shuttle vector that can propagate within the cells of E.coli and yeast. Another is the adenovirus shuttle vector that can propagate in E.coli and mammal cells.

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Genetic engineering - Help

... before using your primers) Clone it into a appropriate expression vector (should be a shuttle vector that can be expressed in bacteria and eukaryots – that means it contains origin ...

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by Orome
Fri May 07, 2010 8:14 am
Forum: Genetics
Topic: Genetic engineering - Help
Replies: 12
Views: 7036

Some MCQs

... to a recessive genetic disease allele which statement is TRUE: BAC vectors in e.coli are based on the pMBI replicon and replicon and replicate ... number in plant nuclei viral replication origins are used in mammalian shuttle vectors becasue there are no natural plasmids in mammalian cells ...

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by biology_06er
Tue Nov 13, 2007 2:25 am
Forum: General Discussion
Topic: Some MCQs
Replies: 3
Views: 5665

Please explain to me - CMVp

... CMV: constitutive promoter for mammalian expression Do you have a shuttle vector for both bacterial and mammalian expression? The CMV promoter on a cloning plasmid is ...

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by Duke
Tue Oct 02, 2007 11:37 am
Forum: Molecular Biology
Topic: Please explain to me - CMVp
Replies: 4
Views: 4085

Commerial Sources of Plasmids

It seems that pBI 121 is a shuttle vector that was produced by clontech. But they do not seem to carry it anymore. although they seem to carry ...

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by canalon
Sat Jun 23, 2007 2:28 am
Forum: Molecular Biology
Topic: Commerial Sources of Plasmids
Replies: 2
Views: 1630

Plasmid duplication sites

thanks mate So if I use ndele and hindilll enzyme on this vector it will be cut and the bgal gene would be separated from ampicllin and ... marker for the same target anf your favorite gene, you would have build a shuttle vector.

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by canalon
Sat Jun 16, 2007 9:40 pm
Forum: Cell Biology
Topic: Plasmid duplication sites
Replies: 11
Views: 5762
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