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Shine-dalgarno sequence

shine-dalgarno sequence

A short stretch of nucleotides on a prokaryotic mRNA molecule upstream of the translational start site, that serves to bind to ribosomal RNA and thereby bring the ribosome to the initiation codon on the mRNA.


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A question I'm finding hard to answer (recombinant protein)

... promoters. In designing the expression experiment, a bacterial promoter sequence should be selected that is optimum for the desired expression levels ... first. Concerning translation: Eukaryotic mRNAs do not contain a Shine-Delgarno sequence which is required in bacteria for correct positioning ...

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by ibz52002
Sat Apr 28, 2012 4:45 pm
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 6968

When are parts of prokaryotic operons seperated?

... seperated? Are the mRNA actually spliced? Or does the operon have one Shine-Delgarno sequence for each gene sequence in the mRNA, so that the ribosome can bind on more than 1 place ...

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by poobear
Thu Nov 06, 2008 5:41 pm
 
Forum: Genetics
Topic: When are parts of prokaryotic operons seperated?
Replies: 5
Views: 5397

Re: mRNA help

The Shine-Delgarno sequence aligns the ribosome with the start codon of the message. What effect do you think that will have on the ...

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by blcr11
Mon Mar 10, 2008 1:49 pm
 
Forum: Molecular Biology
Topic: mRNA help
Replies: 1
Views: 1585

mRNA help

If I had a sequence given to me what would happen if the shine dalgano sequence before the start codon in the mRNA was replaced with CTTTCCCTT? Will the no. of molecules ...

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by georgina009
Mon Mar 10, 2008 2:18 am
 
Forum: Molecular Biology
Topic: mRNA help
Replies: 1
Views: 1585

help in identefying a sequence within a sequence

... cause whenever i transfect it into culture cells. they start to shine like the green man from chernobil. my problem is, i want to create ... create a restriction site map.... but... how do i find those functional sequences(such as gfp) inside my plasmid? i tried looking for parts of gfp ...

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by shachtal
Mon Aug 06, 2007 4:37 pm
 
Forum: Molecular Biology
Topic: help in identefying a sequence within a sequence
Replies: 2
Views: 1491
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