Shaker

... that the average barmaid is usually not completely able to understand. LOL, agree. Show me a barmaid who does not mistake an accelerator for a new shaker.

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... in under 2 hours, since the diameter of the wells are so small and the reader only has one shake setting. Has anyone tried using a regular shaker (8mm, 25mm orbit), and how fast do you have to go to keep the cells in suspension? Thanks, Dan

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That's the only thing I can think of...perhaps if you and two other people take "shifts" to stir? I'm afraid that the motions of any automated shaker/stirrer would also give you this problem. LOL, sometimes, in the interests of carrying out a scientific expirement requires a little sleep deprivation...

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Hi I have been growing and expressing proteins in insect cells for aprox 4 years. When I grow my cells in shaker cultures, I get a 'sludge' ring of cells that stick to the glass flask, just slightly higher than the media level. After a few hours of placing cells in a new flask, ...

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