Serum

I have started using Invitrogen's freestyle CHO cells (without serum, and in suspension) but the protein expression levels are not great compared to the HEKs I was using before. I have played around with ratios and have an optimal ratio (1:1 with 1ug DNA ...

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... bleach, formalin, or glutaraldehyde; buffers with detergents, such as 10% SDS, since they can boil over; heat labile ingredients such as serum, vitamins, antibodies, and proteins; mammalian, plant and insect media; HEPES-containing solutions; DTT or B-ME containing solutions 3. Such un-autoclavable ...

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... absorptivity) of NADH. I'll take your word for it that it is 6.22E3 A/ M cm. What you haven't told anyone is what the volume of your sample of serum was and and if it was a dilution that was measured. What you've calculated (almost, anyway) is the concentration (in moles per L or molarity) ...

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... 6.22*10^3l.mol^-1cm^-1 now I have started this by rearranging the beer lambert equation so c = A / E *l so for example one of my readings was for serum = 0.854 so i did c = 0.854 / (6.22*1) = 0.137 and I am stuck there. I am not sure what units is the results and what i do to convert it to micromols?? ...

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... rhythm of 80 beats/minute ON ITS OWN, without any nerves (it still does that if you take the heart out of the body and put in a bowl of nutritive serum. This is due to special self-depolarizing cells called pacemaker cells, and particularly to special sodium channels in the plasma membrane of ...

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