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Plural of serum.

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Dilution-titrate 2 fold definition

Hi there, I have some sera and if it says "dilute sera in PBS-T (titrate 2 fold) starting at 1:200...what exactly does it mean... I'm thinking say I start with a 1:200 1mL sample...take 5uL of sera and add 995uL of PBS-T..that would ...

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by biology_06er
Tue Nov 22, 2011 9:16 pm
Forum: General Discussion
Topic: Dilution-titrate 2 fold definition
Replies: 1
Views: 1869

What does this mean??? Please help

... to Functional Subclasses of t lymphocytes bearing different Ly Antigens. I do not understand what is being done to each cell? I know that antisera is a fluid phase of clotted blood that contains specific antibodies against the immunizing antigen but was is the immunizing antigen in this case? ...

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by speakers2
Sun Oct 16, 2011 1:06 am
Forum: General Discussion
Topic: What does this mean??? Please help
Replies: 0
Views: 2579

Why do different batch of reagents produce different results

... you never really know what all the serum etc. actually contains, since every individual is different. So they make one batch where they mix sera of different individuals and all units of that batch are similar. But then they make a new batch and they've obtained it from different individuals ...

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by biohazard
Fri Dec 18, 2009 8:24 am
Forum: General Discussion
Topic: Why do different batch of reagents produce different results
Replies: 4
Views: 1848

Re: Matching 2D membrane spots to the gel

... an one biological sample. I make the Western blotting with nitrocellulose membrane and first POLYclonal antibody. For second antibody we use SWAR sera. After this proccess I stain the nitrocellulose with PONCEAU and then I choose some place of membrane. In my case 30-75 kDa. I display spots of ...

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by JackBean
Mon Sep 21, 2009 8:39 pm
Forum: Molecular Biology
Topic: Matching 2D membrane spots to the gel
Replies: 1
Views: 1852

doble fertilization-first genus

Well... que sera sera :lol: Nah, seriously, I still wanna know about this, makes me curious :)

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by Dr.Stein
Mon Aug 15, 2005 12:55 am
Forum: Botany Discussion
Topic: doble fertilization-first genus
Replies: 8
Views: 4530
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