Sequences

OK, so you have DNA sequences from related species which are somewhere diverse, somewhere are identical. For example the region coding for active site will be probably conserved, while some loops on surface of the protein will be divergent. ...

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Because if the amino acids are identical, the DNA sequences are probably also very similar (but does not have to be identical!), thus primer annealing to this region may be used for several species. It can be used either as unspecific primer for known ...

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... functioned as the catalysts for cell cycle progression. To isolate a cDNA encoding a CDK in maize, investigators aligned the predicted amino acid sequences of human cdc2, S. pombe cdc2, and S. cerevisiae cdc28 genes. Degenerate oligonucleotide PCR primers corresponding to the boxed amino acids ...

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That depends whether you add some homology sequences and homology recombination will be used to incorporate that gene. Honestly, I don't know, what's the ratio of HR and NHEJ in human.

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... therefore it is not Duchenne muscular dystrophy (DMD) and is not a mutation of the dystrophin gene. Thanks, I had missed that. The upper sequences look identical, while the lower sequences show two different sequences for the female (due to the N position, which shows two traces) and ...

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