Sequence

the Kozak sequence is around the START codon, so you've had to play with the vector. But it's probably normally working if they sell it in Life Technologies.

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... biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? So where should I add the Kozak ...

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... cell the mtDNA should be relatively conserved. However, the mutational rate of mtDNA is ten fold higher than that of nuclear DNA. So is the mtDNA sequence conserved among e.g. various mammalian species or the opposite?

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OK, so you have DNA sequences from related species which are somewhere diverse, somewhere are identical. For example the region coding for active site will be probably conserved, while some loops on surface of the protein will be divergent. ...

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... functioned as the catalysts for cell cycle progression. To isolate a cDNA encoding a CDK in maize, investigators aligned the predicted amino acid sequences of human cdc2, S. pombe cdc2, and S. cerevisiae cdc28 genes. Degenerate oligonucleotide PCR primers corresponding to the boxed amino acids ...

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