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Sequence

sequence

The noun: the order in which subunits appear in a chain, such as amino acids in a polypeptide or nucleotide bases in a dNA or rNA molecule.

The verb: To find out in what order the subunits appear in the chain.


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Help: Confused with primers in a PCR of a vector?

... I think the point of this entire process was to just determine the directionality of our insert. Because later in the lab, we did a whole bunch of sequence alignments with our gene (of both strands) and BLASTed it. But...I am just not really sure if I've done the PCR reactions correctly. Any help ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

cladograms

Here is a task: Look at the picture - you see three DNA sequences from three species that are related. Based on that sequence only, draw a cladogram that best represents their relatedness. http://i59.tinypic.com/2jbmgax.jpg My thoughts: Since species ...

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by leeroykincaid
Tue Feb 25, 2014 8:09 pm
 
Forum: Evolution
Topic: cladograms
Replies: 7
Views: 315

Re: Annealing Temparature

Primer_F: 5'-GCTCTAGAGTGATAGTGAGCATGG-3' Primer_R: 5'-GCGATATCCTACACCCAAGCTGC-3' CORRECTED PRIMER SEQUENCE. THANKS ALL. I WILL TRY SUGGESTED EXP. CONDITION. THANKS "vladCH".

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by Helics
Sun Feb 23, 2014 4:59 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350

Free online software tools for molecular cloning

... website http://www.cloninghelp.com where you can find a couple of browser-based tools facilitating your everyday cloning work. At this time a DNA sequence editor and a restriction analysis tool are available. I have also some other ideas that may evolve into additional tools in future. I will ...

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by vladCH
Sat Feb 22, 2014 8:12 pm
 
Forum: Molecular Biology
Topic: Free online software tools for molecular cloning
Replies: 0
Views: 139

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 15557
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