Dictionary » S » Sequence

Sequence

sequence

The noun: the order in which subunits appear in a chain, such as amino acids in a polypeptide or nucleotide bases in a dNA or rNA molecule.

The verb: To find out in what order the subunits appear in the chain.


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exon intron junction

... Do you guys know other software that can predict exon-intron junction apart from GENESCAN, NetGene, FGENESH and RNASPL? I also had tried multiple sequence alignment but I need other software to strengthen my result. Unfortunately the software above are not enough. Thank you in advance.

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by elynn
Tue Sep 09, 2014 6:48 am
 
Forum: Molecular Biology
Topic: exon intron junction
Replies: 0
Views: 45

5' LTR sequece

Hi, I want to check the 5' LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5' LTR and not a wild type HIV-1 5' LTR. Can someone pls guide me on how I can evaluate this? Best, John

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by aguy
Mon Jul 07, 2014 10:02 pm
 
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 1
Views: 373

Re: Is DNA living or non-living thing?? get the answer here

... this mass-energy dualism also the quantity of energy in the universe is constant. Since the last big-bang all mass formats undergo the same cyclic sequence like the universe itself i.e. birth – evolution – survival – replication. In order to survive and to repeat this sequence every mass format ...

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by Dov Henis
Fri Jul 04, 2014 8:00 am
 
Forum: Molecular Biology
Topic: Is DNA living or non-living thing?? get the answer here
Replies: 24
Views: 54661

Mutagenesis

http://www.ncbi.nlm.nih.gov/pubmed/20677001 First you use a sequence with the selection marker (as usually), but that is later excised by I-SceI cleavage and the second Red recombination.

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by JackBean
Sun May 11, 2014 6:35 pm
 
Forum: Genetics
Topic: Mutagenesis
Replies: 2
Views: 741

Help: Confused with primers in a PCR of a vector?

... I think the point of this entire process was to just determine the directionality of our insert. Because later in the lab, we did a whole bunch of sequence alignments with our gene (of both strands) and BLASTed it. But...I am just not really sure if I've done the PCR reactions correctly. Any help ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2428
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