Sephadex

I'd have to see the method. Are you sure you meant Sephadex G10? Sepahadex is a sizing resin. Its two primary uses are for clean up of protein preps usually as a final or near-final step, or separation of nucleotides and salts from a DNA or RNA reaction ...

See entire post

I want to get a pure populaiton of pre-osteoclasts from mouse bone marrow cells. I've read papers uing Sephadex G-10 column to purify bone marrow cells to get pure pre-osteoclasts. Does anybody know how this column work? How can it deplet other cell tpyes? THANKS A LOT!

See entire post

... and normal rats was obtained. Incubation conditions, the effect of salts and chelating agents, the chromatographic behavior in DEAE-cellulose and Sephadex G-100 columns, were comparatively studied with both enzymatic preparations. The effect of sodium diethyldithiocarbamate, sodium pyrophosphate, ...

See entire post

use some kind fo gel chromatography, sephadex column is most common.

See entire post