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Dictionary » S » Separation

Separation

separation

The act of separating, or the state of being separated, or separate. Specifically:

chemical analysis.

divorce. The operation of removing water from steam. Judicial separation, a form of divorce; a separation of man and wife which has the effect of making each a single person for all legal purposes but without ability to contract a new marriage.

Origin: L. Separatio: cf. F. Separation.


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Re: Animal cell nucleus shape changes

... centrifugal force. It can be write as RCF = rw2/g where = angular velocity = rotational radius g = Gravitational acceleration The Centrifugal separation methods are of three types Differential pelleting Rate zonal centrifugation Isopycnic centrifugation In differential pelleting a sample is ...

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by andrewpasha
Tue Nov 20, 2012 3:37 pm
 
Forum: Cell Biology
Topic: Animal cell nucleus shape changes
Replies: 7
Views: 2203

Re: separation problem with GelRed

Sadly I did not help us out. In our lab often we want to check early if we have good product/restriction pattern. And with GelRed gels have to run longer to get nice bands. But it helps indeed if you run longer. Then result can be nice.

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by citroenboom
Wed Nov 14, 2012 1:41 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 11
Views: 12141

Re: separation problem with GelRed

I got the tip from another lab to dilute the DNA ladder and now it looks so much better, nice separation. We have diluted the ladder 1:10. Good for the lab-economy as well! Maybe one could go further even, and also dilute the markers- have not tried this yet. When I looked ...

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by annakarinring
Tue Oct 30, 2012 12:50 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 11
Views: 12141

Re: aneuploidy and polyploidy

... and trisomy) and it can occur in sex chromosomes or autosomes...AND there are different causes (non-disjunction, translocation errors, mitosis separation failures in early embryos). Plus there are a whole range of syndromes associated with each of these types (i.e. Jacob Syndrome is an aneuploid ...

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by carmodyf
Thu Oct 11, 2012 4:36 am
 
Forum: Cell Biology
Topic: aneuploidy and polyploidy
Replies: 2
Views: 1395

Re: separation problem with GelRed

Hi there, after few tests, the similar problem still exist, especially for the ladder. my lab uses both 1% and 2% gel. 1x TAE for dissolving and also running. 100v for 50mins. I have tried using low voltage and higher voltage as well, yet the output still the same. Any suggestion or advise? Thank y...

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by citroenboom
Thu Jun 14, 2012 11:44 am
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 11
Views: 12141
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