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Secondary

secondary

second or inferior in order of time, place or importance, derived from or consequent to a primary event or thing.

Origin: L. Secundarius, secundus = second


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Re: Difference between ICC and IF

... of a name. However, you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 7526

the harm of algae

This is a very broad question, do you mean the harmful effects of harmful algae blooms, specific algae species e.g. pseudo-nitzschia produces a secondary metabolite called domoic acid which is a neurotoxin and bio accumulates in marine life causing respiratory problems and can lead to amnesiac ...

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by marineman
Sun Feb 24, 2013 1:28 pm
 
Forum: Botany Discussion
Topic: the harm of algae
Replies: 1
Views: 481

Rhodophycae, Secoundary metabolites effects on parasite load

... which species of red algae to choose. Does anyone know which species (located in the uk- preferably in south west of England) which would produce secondary metabolites which would effect the parasite loading of an organism, such as a-kainic acid or domoic acid ? Thanks !

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by marineman
Sun Feb 24, 2013 12:59 pm
 
Forum: Botany Discussion
Topic: Rhodophycae, Secoundary metabolites effects on parasite load
Replies: 0
Views: 52

How can i asses the parasie loading of littorina littoria?

I was hoping to assess the effect of secondary metabolites of several species of Rhodophycae to see if it produces an anti-parasitic response so i kinda need them to be as undamaged as possible. The trematodes would be located in the foot and ...

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by marineman
Fri Feb 22, 2013 6:38 pm
 
Forum: Zoology Discussion
Topic: How can i asses the parasie loading of littorina littoria?
Replies: 7
Views: 366

Re: Difference between ICC and IF

Typically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for every antigen ...

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by jonmoulton
Wed Feb 20, 2013 5:20 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 7526
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