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Sec

Sec

1. Abbreviation for selenocycsteine. Insertion into selenoenzymes is accomplished by the specific tRNA(sec).

2. E. Coli complex for translocation of proteins across the inner membrane, mutants SecA, SecY.


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Campylobacter RFLP troubleshooting

Wait a sec. So you amplify your GOI and then cut it with RE for what exactly? Just at the ends to make sticky ends for consequent cloning? And I guess you use your PCR reaction without any purification?

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by JackBean
Sun May 05, 2013 3:54 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1910

Do We Have The Freedom Of Choice?

@biohazard: Let's forget about the free will for a sec and let's talk about consciousness. I guess one neuron is not aware of itself, is it? Now, let's take bunch of neurons, will they have consciousness? If so, when did they get it? After we merged ...

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by JackBean
Sun Apr 07, 2013 8:02 pm
 
Forum: Cell Biology
Topic: Do We Have The Freedom Of Choice?
Replies: 27
Views: 27172

Primers Tm

... time since they amplify almost the same size of a product which is around 240 bp. I am using KOD-Plus kit. cycling conditions are 94/2 min 94/15 sec 55/ 30 sec 68/ 15 sec I got faint bands but at the same time it was smeared, I am using the optimal reaction volume from the kit and I tried it ...

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by bravebeaker
Thu Oct 11, 2012 7:18 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 2775

Re: Why use trypsin in cell cultures?

... them to your new cell culture dish with fresh nutrients. In general, due to its protease function, trypsin shouldn't be left for more than 30 sec in contact with your cell culture otherwise your cells will be teared apart and I guess you don't want this to happen. Cheers, Katerina

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by kafrin313
Thu Aug 11, 2011 10:15 am
 
Forum: Molecular Biology
Topic: Why use trypsin in cell cultures?
Replies: 1
Views: 12391

heart - systole/diastole on graph?

... given an assignment to label the following graph with atrial systole, atrial diastole, ventricular systole and ventricular diastole. They are gram/sec graphs measured on frog heart. Can someone help me with it? I cannot decide whether it is starting with atrial or ventricular ones.. Thank you from ...

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by smoutera
Sun Mar 27, 2011 5:01 pm
 
Forum: Physiology
Topic: heart - systole/diastole on graph?
Replies: 0
Views: 3073
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