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Sea needle

sea needle

(Science: zoology) See garfish .


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western blotting of DNA-binding protein of mammalian cell

... in 25 cm2 flask and collected the cell lysate using RIPA lysis (1 ml) buffer...kept in ice for 5 min, followed by passing the lysate through 24 G needle about 5 times...then centrifuged at 12000 g for 15 min..collected the supernatant and pellet.. from the supernatant 40 ul of sample taken and ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 394

Re:

... its concentration gradualy decreases. At near bottom there is no oxygen at all. If we add suitable nutrients in the agar and innoculate with a needle (stab innoculation) after suitable incubation time we can know microbe's oxygen requirements. Aerophile grow on the top, microaerophile in middle ...

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by sosippus
Wed Sep 24, 2014 7:44 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 7
Views: 4470

Oxygen requirements of eukaryotes

... its concentration gradualy decreases. At near bottom there is no oxygen at all. If we add suitable nutrients in the agar and innoculate with a needle (stab innoculation) after suitable incubation time we can know microbe's oxygen requirements. Aerophile grow on the top, microaerophile in middle ...

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by josem
Wed Sep 24, 2014 5:28 pm
 
Forum: Microbiology
Topic: Oxygen requirements of eukaryotes
Replies: 7
Views: 4470

Help! I'm freaking out so badly please answer this!

sometimes I get these 'hot needle' sensations on my skin & it feels hot can I catch fire from this, I'm so scared..I usually get it on my head and legs. I read in the news that its possible for your cells to heat up so rapidly your ...

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by Coelacanth
Thu Jan 02, 2014 9:08 am
 
Forum: General Discussion
Topic: Help! I'm freaking out so badly please answer this!
Replies: 14
Views: 7437

Re: Re:

... of blood cells in the sample (billions of them) totally overwhelms the few bacteria that may end up in the sample from the skin, and because the needle is very sharp. For blood culture purposes the skin is also carefully disinfected, but even that does not remove the bacteria from the deep layers ...

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by snowathlete
Fri Dec 13, 2013 2:08 pm
 
Forum: Microbiology
Topic: What do you think these are, in my blood?
Replies: 16
Views: 15882
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