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Screening

screening

examination of people with no symptoms, to detect unsuspected disease.


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Help: Confused with primers in a PCR of a vector?

... what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

TLDA screening analysis

Hello, I am looking for a software to analyze a large number of files from a screening analysis of TLDA. We have a Workflow in R for analyzing exported .txt files, but we haven't software to analyze all the plates at once in order to analyze the amplification ...

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by laurentPre
Fri Jan 17, 2014 12:59 pm
 
Forum: Molecular Biology
Topic: TLDA screening analysis
Replies: 4
Views: 470

blank and substrate control give OD reads

Hi there...I'm new here. Hopefully all of u can solve my problem. I'm working on screening of xylanase variants to look for the better variant than the wild type in hydrolysis kenaf. the reaction is done in 96 deep well plate by adding substrate (kenaf) + buffer ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1159

question about G418 sceening test

... new to cell culture experiment, so I'm looking for someone who can help me out here. I'm trying to determine a threshold concentration for G418 screening of stable transfected cells (Huh-7). I start with a T25 flask of Huh-7 about 80% confluence, and passed it to a 6 well plate, each is about ...

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by cavaliersun2003
Mon Nov 11, 2013 7:28 am
 
Forum: Cell Biology
Topic: question about G418 sceening test
Replies: 0
Views: 568

aflatoxin screening Test and peaunt allergen screening test

aflatoxin screening Test what substance is found in the red wells and the clear wells provided? Ans : red well is enzyme and clear wells is antibody? <---- is that right peaunt allergen screening test what substance is found ...

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by Damian2012
Fri Oct 04, 2013 5:11 pm
 
Forum: Human Biology
Topic: aflatoxin screening Test and peaunt allergen screening test
Replies: 2
Views: 509
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