Screening

... so we are trying to avoid that route. If we try to, for example, use linear DNA without some selectable marker, we'll just have a difficult time screening for our mutant. One interesting detail: the GC content of the upstream and downstream flanking regions used to recombine with the flanking ...

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elynn: in such case it would be probably best to prepare slightly degenerated probes for whole length and do some screening of libraries.

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which gene from what organism? Do you know the gene sequence? If not, you will have to try either degenerated primers or screening genomic/cDNA library or something like that.

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I understand, thank you very much for the clarifications.

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Hi, The concept of Synthetic-lethality and Synthetic Genetic Array have been introduced to us in class recently, but I'm struggling with understanding it. Quite frankly, I don't even understand what questions one is seeking answer to using these techniques. I was given some papers to read up on so t...

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